BIO205 - Ch 3 -Observing Microorg. Through Microscope - RioSalado - AZ
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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| show | 10^-6 m
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| prefix micro | show 🗑
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| nanometer (nm) | show 🗑
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| Quorum | show 🗑
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| show | illuminator, condenser
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| show | objective lenses
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| eyepiece | show 🗑
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| show | Objective lense magnification (power) times the ocular lense magnification (power).
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| Resolution | show 🗑
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| show | shorter
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| The white light in compound light microscope has __ wavelength. | show 🗑
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| show | 2000x
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| Refractive index | show 🗑
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| show | By staining them - they will then have different refractive indexes.
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| show | refractive index - keeps light rays from refracting as they enter air - increases resolving power of lenses.
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| Brightfield illumination | show 🗑
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| show | Used for invisible microorganisms & cannot be stained - uses darfkield condenser with opaque disk - sees only reflected light - black background.
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| show | Good to examine interior cell structures - Used with living microorganisms - don't have to fix them - uses special condenser to absorb refracted light & interference patterns.
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| Differential interference contrast (DIC) microscopy | show 🗑
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| Fluorescense | show 🗑
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| Fluorescence microscopy | show 🗑
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| Principal use of fluorescence microscopy | show 🗑
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| show | 3D image made by light microscope - uses fluorescent stains & laser - computer constructs image from stack of images - ATP & Ca ion concentrations.
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| Scanning Acoustic Microscopy (SAM) | show 🗑
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| show | Use of pinhole aperture
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| show | Study living cells attached to another surface - cancer, plaque, etc.
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| show | in black & white
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| show | Transmission - thin sections of a specimen & scanned - 3d view of surface.
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| Positive staining | show 🗑
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| show | Increase electron opacity of surrounding field & useful when studying very small particles/specimins like virus particles, bacterial flagella, proteins.
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| show | Heavy metals adhere at 45 degree angle on one side & leaves a shadow to crate 3d for size.
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| TEM has __ resolutions & valuable for examining __ of specimen. | show 🗑
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| Drawback of TEM? | show 🗑
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| show | 3d view of surface - good for intact cells & viruses - uses electrons
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| show | Doesn't modify specimen or damage it - map atomic & molecular shapes, characterize magnetic & chemical properties, & temp variation inside cells.
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| show | Thin metal (tungsten) probe scans specimen & produces an image revealing bumps & depressions of the atoms on surface of specimen.
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| show | Metal & diamond probe - 3d image - doesn't damage specimen - both biological substances & molecular processes.
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| Stains are __ composed of positive & negative ion. | show 🗑
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| The color of __ dyes is in the positive ion. | show 🗑
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| show | acidic
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| Name some basic dyes | show 🗑
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| Most commonly used dyes | show 🗑
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| __ dyes are not attracted to most types of bacteria. | show 🗑
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| show | To prepare colorless bacteria against colored background - eosin, acid fuchsin, & nigrosin
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| Simple stain | show 🗑
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| show | Chemical that increases affinity of stain for biospecimen or coats it.
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| Differential stains | show 🗑
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| show | Stain that classifies bacteria into gram-positive & gram negative.
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| 4 steps of gram staining | show 🗑
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| Primary stain | show 🗑
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| Decolorizing agent | show 🗑
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| Gram-positive | show 🗑
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| Gram-negative | show 🗑
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| Counterstains | show 🗑
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| What reacts to create gram stain? | show 🗑
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| show | positive
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| Acid-fast stain | show 🗑
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| show | Tuberculosis & leprosy
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| show | Used to color & isolate special parts of microorganisms & to find capsules.
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| Capsule | show 🗑
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| Virulence | show 🗑
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| Endospore | show 🗑
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