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Cytology Quiz

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1. EA stains metabolically inactive cells?




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2. Problem: bloody specimens:




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3. Bodys fluids pleural, peritoneal, pericardial, ascites treatment:




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4. Pull-apart smear method




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5. Cytocentrifuge smear method




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6. Cell block for specimen w/ loose cellular material - agar method:




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7. Breast/nipple discharges treatment:




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8. Fluids include:




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9. ThinPrep System




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10. Nickel smear method




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11. To remaining sediment add 30% BSA, mix w/ stick, add 95% alcohol, place mass in cassette, may wrap w/ lens paper, if too large submit portion, formalin process routinely




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12. Commerical cytology sprays - usu. ethanol w/ acetone & polyethylene glycol (remove latter w/ alcohol before staining), 95% denatured alcohol, 80% isopropanol (b/c more shrinkage than ethanol), 100% methanol (b/c less shrinkage than ethanol)




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13. To remaining sediment add 95% ethanol, denatured alcohol, or 10% NBF, mix by inversion, fix 15-30 min, centrifuge 10 min, 2,000 rpm, pour off supernatant, mass in cassette, may wrap w/ lens paper, if large submit portion, formalin process routinely




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14. Urine, sputum, CSF, bronchial/gastric/esophageal washings or brushings, pleural/peritoneal/ascites/pericardial fluids, FNAs, best collect fresh w/ no fixative/additive, most can be refrigerated 24-72 hrs, don't freeze




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15. Cytocentrifuge




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16. Ongoing concern, cells may float into reagents or stick to tools, change reagents between samples & use disposable tools




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17. 95% ethanol, rapid fixation important to prevent distortion of cell & loss of morphology,




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18. Sample w/ not many cells in suspension can be made more conc'd by Cytospin or centrifugation preparation: 1mL properly prepared cell suspension in funnel of cytospin, centrifuge, cells will be deposited on slide & fluid absorbed by filter paper




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19. The act of preparing & staining diagnostic slides




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20. V. fragile, doesn't hold pH, first morning urine not recommended b/c cells degenerated, add pre-fixative if can't process or send to lab right away, equal volume of alcoholic saline or Sacomanno fluid





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