The most important step in the histology laboratory
fixation
pH of the staining sollution
cells have specific affinity for stains/dyes with specific pH ranges
Formaldehyde is colorless ________.
gas
Alcian Blue solution with a pH of 2.5
stains CARBOLYLATED mucins in connective tissues and cartilage.
Stabilization of proteins is done in order to prevent
tissue destruction during subsequent steps in processing
The rate of penetration is NOT affected by _______.
the concentration of the fixative
PAS with Diastase
used in conjunction with the PAS staining procedure to specifically identify glycogen granules in tissue samples. Amylase enzymes in a malt extract are used to digest the glycogen, which is then washed out of the tissue sample.
TEMPERATURE of the dye
increased temperature = increased rate of diffusion throughout the tissue
