Question | Answer |
1. THE PURPOSE OF A FIXATIVE IS TO | Harden tissue for further processing |
2. THE TERM DENATURE APPLIES TO | Protein |
3. A "PLANOCONCAVE" MICROTOME KNIFE MAY BE DEFINED AS: | Having one side concave and one side straight |
4. THE PROPER ORDER OF TISSUE PROCESSING IS: | Fixation, decalcification, dehydration, clearing and embedding |
5. The clearing stage of tissue processing: | Is an intermediate step between alcohols and paraffin.
Is an intermediate step between dehydrating reagents and embedding media
Can be dne with exylene, toluene, chloroform or benzene |
6. FIXATIVE CAN INCLUDE: | Helly's Zenker's and Boulin's |
7.WHEN DEALING WITH PICRIC ACID: | Always keep the picric acid wet |
8. A TEST FOR DECALCIFICATION COULD BE: | K ion determinations |
9. THE MOST COMMON FORM OF EMBEDDING MEDIA IS: | Paraffin wax |
10. WHEN DECALCIFYING BONE THE VOLUME OF FLUID USED PER VOLUME OF TISSUE IS: | 20x |
11. PUTREFACTION IS THE SAME AS: | Bacterial decomposition |
12. MOST ROUTINE MICROTOMY IS PERFORMED ON THIS TYPE OF MICROTOME | Rotary |
13. THE ANGLE OF CLEARANCE IS: | The angle made by the block face and the bevel of the knife |
14. TISSUE SECTIONS WITH A CALCIFIED SURFACE SHOULD BE PLACED SO THAT: | The knife passes over this part last |
15. WHEN EMBEDDING MULTIPLE TISSUE SAMPLES IN ONE MOLD: | The pieces of tissue should touch each other |
16. MOST FIXATIVE ARE USED AT: | Room temperature |
17. ADVANTAGES OF FORMALIN FIXATION INCLUDE: | No excessive hardening, Inexpensive, Good penetration |
18. DISADVANTAGES OF FORMALINE FIXATION INCLUDE: | Irritating odor,Reduction in PAS positively |
19. TO PREVENT ACID FORMALDEHYDE HAEMATEIN: | Use buffered formalin |
20. TO REMOVE MERCURY PIGMENT: | Use iodine and sodium thiosulfate alcoholic iodine |
21. PICRATE ARTIFACTS ARE not DUE TO: | Chromium fixative being present, precipitation of mercuric chloride, production of picrate dichloride |
22. LARGE PIECES OF BRAIN: | Are often embedded in cellulose nitrate |
23. A DISADVANTAGE OF PARAFFIN WAX EMBEDDING IS: | Inabilit to cut very thin sections |
24. FIXATION: | Alters the way tissues bend light, Is an artifact, Prevents autolysis |
25. A STABILIZER FOR A FORMALIN FIXATIVE IS: | Methanol |
26. A 5% SOLUTION OF FORMALIN does not CONTAIN: | 2%, 4%, 5%, 10%, formaldehyde |
27. FORMALIN | Preserves lipids well |
28. PINK DISEASE IS SEEN IN: | Formalin fixed tissue wiwth H&E stain |
29. ZENKER'S FLUID CONTAINS: | Mercuric chloride and acetic acid |
30. CARNOY'S FLUID CAUSES: | Shrinkage
Lysis of red cells
Preservation of glycogen |
31. THE APPROPRIATE FIXATIVE FOR BONE MARROW PREPS AND BLOOD SMEARS IS: | Alcohol |
32. THE MORE DELICATE A TISSUE IS THE: | Lower the % of alcohol it needs |
33. THE MELTING POINT OF PARAFFIN WAX: | Is higher as the wax gets harder |
34. VACUME IMPREGNATION: | Is not required for tissue such as spleen, Is not recommended for cysts. Does not require a longer fixation time. |
35. TISSUE IMBEDDED IN CELLULOSE NITRATE: | Requires storage in alcohol
Will not ribbon
Are usually very hard |
36. DECALCIFICATION: | Is best done in a 25 degrees celcius water bath |
37. THE APPROPRIATE VOLUME OF EDTA IS_____OF THE TISSUE VOLUME: | 150% |
38. TRICHLOROACETIC ACID SHOULD BE USED IN A CONCENTRATION OF ___% WHILE FORMIC ACID SHOULD BE AT ___% | 5/10 |
39. THE FIRST STEP IN SHARPENING A KNIFE IS )___ AND THE KNIFE EDGE COMES ____ | honing / first |
40. TO ADHERE THE TISSUE SECTION TO THE SLIDE ____ | Albumin |
41. IN AN H & E STAIN: | The nuclei are blue |
42. DRYING TISSUE SECTION SHOULD BE AT ___FOR ___HOURS | 37 degree celcius / 16 |
43. THE MOST COMMON MORDANT FOR H & E IS: | Na metabisulphite |
44. MORDANTS ARE: | Necessary for tissue staining |
45.THE DECOLORIZER FOR H & E IS ___AND IT WORKS BY ___ | Acid-alcohol / breaking the lake |
46. ARTIFICIAL RIPENING AGENTS INCLUDE: | Na iodate and K permanganate |
47. TAKING SECTIONS TO WATER REQUIRES: | Clearing and rehydrating |