Question | Answer |
Chicken embryo culture | historic method for viral culture, technically difficult and labor intensive |
Cell types used for culture | Primary, diploid, continuous |
Primary cell lines | derived directly from donor, rhesus monkey or rabbit kidney, 1-2 passages |
Diploid cell lines | Prepared from animal tissue, lung or foreskin fibroblast, 20-50 passages, terminally differentiated-post mitotic |
Continuous cell lines | single cell type propogated indefinately, derived from tumor cells, unlimited passage. A549 (human lung carcinoma), HEp-2 |
Cytopathic effect | Rounding up or detachment, lysis, nuclear swelling, cell fusion (syncytia) |
Hemadsorption | hemagglutinin HA produced by some viruses binds erythrocytes, uses human type O, guinnea pig, chicken, performed on culture from resp samples |
Fluorescent ab test | confirms CPE, some direct samples, CMV, HSV, respiratory viruses, enteroviruses |
Shell vial culture | cells on coverslip in shell, can have multiple cell lines, centrifuged, faster results, use for CMV, HSV, ZVZ, chlamydia, resp viruses |