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The primary targets for microbial control are
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Which microbes have the highest RESISTANCE?
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The primary targets for microbial control are microorganisms capable of causing infection or spoilage (that are constantly present in the external environment & on the human body
Which microbes have the highest RESISTANCE? Prions and bacterial endospores
What is sterilization? A process that destroys or removes all viable microorganisms, including viruses
Can an item be almost sterile? Things can only be sterile or not sterile
The use of a physical process or chemical agent to destroy vegetative pathogens but not bacterial endospores disinfection
the growth of microorganisms in the blood and other tissues SEPSIS
Any practice which prevents the entry of infectious agents into sterile tissues and thus prevents infection Asepsis
A ____________ is normally only used on INANIMATE objects because the concentrations required to be effective can be toxic to human/animal tissue disinfectant
These are examples of _________________: Applying a solution of 5% bleach to an examining table, boiling food utensils disinfection
Aseptic techniques commonly practiced in health care consist of _____________ being applied directly to exposed body surfaces antiseptics
Swabbing an open root canal with hydrogren peroxide is an example of _______ antisepsis (aseptic technique)
Does a -cidal agent result in sterilization? Not necessarily
a cleasing technique that mechanically removes microorganisms and other debris to reduce contamination to organisms sanitization,
Soap and detergent are examples of sanitizers
the reduction of the number of microbes on the human skin involving scrubbing skin or immersing in chemicals degermation
The permanent termination of an organism's vital processes death
How do we determine if microbial death has occured? The most practical way is to determine if a microbial cel can still reproduce when exposed to a suitable environment
Factors that influence rate at which microbes are killed by antimicrobial agents 1. lenght of exposure to agent. 2. effect of the microbial load. 3. relative resistance of spores vs vegetative forms 4. action of the agent, whether microbicial or microbistatic
The cellular targets of physical & chemical agents fall into 4 general categories 1. the cell wall 2. cell membrane 3. cellular synthetic processes (DNA, RNA) 4. proteins
Particularly in gram-negative bacteria __________ & ___________ disrupt the cell walls making them fragile and lysed more easily. Detergents, Alcohol
Chemical agents damage the cell wall by blocking its synthesis, digesting it, or breaking down its surface
Microorganisms have a cell membrane composed of ______________ and ______________ lipids and proteins
large, mixed collection of microbes adapted to the body; includes bacteria fungi, protozoa. Normal flora
some sites that harbor normal flora are skin, upper respiratory tract, GI tract, external genitalia, external ear/eye
Give an example of normal flora Escherichia coli living in the intestine which produce Vitamin K
Not always present, not permanent type of flora Transient
When one microbe benefits, but its cohabitant is neither harmed nor benefited commensalism
exists when organisms live in an obligatory, but mutually beneficial relationship. There is one obligatory dependent but both benefit mutualism
What is viremia? Presence of viruses in the blood stream
The entry, establishment and multiplication of pathogenic organisms within a host Infection
What is a secondary infection? an infection that compunds a preexisting infection
When does naturation occur? when the bonds that maintain the secondary and tertiary structure of the protein are broken
What is a way a protein can be denatured? Through coagulation by moist heat, chemicals such as strong organic solvents, and phenolics
Methods of controlling microorganisms can be divided into two broad categories _______ & _______ Physical and chemical control
Methods of physical control Heat, radiation filtration ultrasonic waves, and even cold, but heat is the most prominent way to physically control microoganisms
The mechanical removal of most microbes from an animate or inanimate surface, but doesn't kill Decontamination
What are the contaminants that need to be controlled? bacterial vegetative cells & endospores; fungal hyphae & spores; yeasts; protozoan trophozoites & cysts; worms; viruses; prions
disinfection of LIVING things Antisepsis
give an example of asepsis handwashing, hydrogen peroxide, iodine
stasis and static mean to stand still; not change
prevent the growth of bacteria bacteristatic
inhibit fungal growth fungistatic
Factors that influence the action of antimicrobial agents # of organisms (load); nature of microorganisms in population; temp & pH of environment; concentration of agent; mode of action of agent; presence of solvents
Antimicrobial modes of action ** the cell wall; the cell membrane; DNA, RNA; proteins (denaturation by alcohols, acids, antibiotics
another name for detergent surfactant
what does a surfactant do? breaks up the lipids
methods of physical microbial control heat - moist or dry
the shortest length of time required to kill all test microbes at a specified temp thermal death time (TDT)
the lowest temp required to kill all microbes in a sample in 10 minutes thermal death point (TDP)
Autoclave uses steam under pressure
most efficient pressure-temperature combination for sterilization 15 psi above normal atmospheric pressure which yeilds 121 degrees
nonpressurized steam tyndallization or intermittent sterilization
used to disinfect beverages pasteurization
two types of pasteurization flash method and batch method
T/F pasteurization does not kill endospores or thermoduric microbes True
What is the simplest way to disinfect? boiling water
ignites and reduces microbes to ash and gas incineration
dehydration of vegetative cells when directly exposed to normal room air desiccation
making beef jerky is an example of desiccation
a combination of freezing and drying; used to preserve microorganisms and other cells in a viable state for many years lyophilization
What is the most common target for radiation? DNA
types of radiation ionizing and nonionizing
technique for removing microbes from air and liquids filtration
What is a tinture? solution dissolved in pure alcohol or water-alcohol mixtures
What are the two branches of the immune system? innate (born with it) and acquired/adapted
The innate branch breaks down into ________ & ______________ first line of defense - physical barrier, chemical barriers, and genetic components & the second line of defense - inflammatory response, interferons, phagocytosis, and complement
The acquired branch or third line of defense can be broken down into _________ and _______ naturally acquired and artificially acquired
Created by: microwoman
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