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Phys Lect 16
Question | Answer |
---|---|
3 ways to remove Ca2+ from cytosol? | 1.Ca ATPase. 2.Na-Ca Antiporter. 3.SERCA Pump |
Steps for relaxation in smooth muscle | 1.Remove Ca2+ from cytosol. 2.Dephosphorylation of myosin head by MLCP (Inc by PKG or PKA). 3. |
MLC Phosphorylation in Phasic contractions | MLCK and MLCP are phos/dephos the myosin head cross-bridges with every contraction. **Ca2+ levels also go up and down with each contraction |
Tonic contraction: Latch state | is a way for smooth muscle to conserve ATP by dephosphorylating the MLC while the myosin head is still forming the cross-bridge. This maintains force through cross-bridges while decreasing ATP hydrolysis. **Can economically stay contracted much longer |
Velocity of contraction during Latch State | 0. b/c the ATP hydrolysis has been reduced, the cross-bridge cycling of normal isotonic contractions will slow making the rate of cycling 0. **Latch State only maintains FORCE, no velocity |
Intracellular [Ca2+] and cross-bridge phosphorylation in Tonic Vs Phasic contractions | 1.Phasic: Both cycle up and down with AP and contraction. 2.Tonic: Ca2+ and c-b phosphorylation go up with AP, but do not return back to base line when the AP leaves. This allows a longer, static force generation. |
If an increase in MLCK? MLCP? | 1.MLCK: Net Phosphorylation of MLC. 2.MLCP: net dephosphorylation of MLC. |
Regulation of smooth muscle contraction | Since initiation is a 2 step process, either of the 2 steps can be regulated: 1.[Ca2+]. 2.MLCK/MLCP |
Main secondary messanger invovled in smooth muscle hormone induced relaxation | cAMP |
Main secondary messanger invovled in smooth muscle hormone induced contraction | IP3 |
Epi/Norepi: Smooth Muscle contraction | 1.Receptor: Alpha1. 2.Secondary Messanger: IP3 |
Epi/Norepi: Smooth Muscle relaxation | 1.Receptor: Beta2. 2.Secondary Messanger: cAMP |
ACh: Smooth Muscle contraction | 1.Receptor: mAChR 2.Secondary Messanger: IP3 |
ACh: Smooth Muscle relaxation | 1.Receptor: mAChR/EC 2.Secondary Messanger: NO |
Angiotensin II: Smooth muscle contraction | 1.Receptor: AT1 2.Secondary Messanger: IP3 |
Vasopressin: Smooth muscle contraction | 1.Receptor: V1 2.Secondary Messanger: IP3 |
Endothelin: Smooth Muscle contraction | 1.Receptor: ETa 2.Secondary Messanger: IP3 |
Adenosine: Smooth muscle relaxation | 1.Receptor: A2 2.Secondary Messanger: cAMP |
PKA | Inhibits MLCK, which will induce smooth muscle relaxation. |
PKC | Inhibits MLCP, which will induce smooth muscle contraction. |
PKG | ACTIVATES MLCP, which will induce smooth muscle relaxation. |
Endothelial cells' effects on vascular smooth muscle | Endothelial cells release several hormones that alter the smooth muscle: 1.NO: relaxtion/ vasodilation. 2.Prostacyclin: relaxation/ vasodilation. 3.Endothelin: conraction/ vasoconstriction. |
Mechanism behind ACh or Bradykinin causing vasodilation | 1.ACh binds to mAChR or EC receptor on endothelial cell. 2.Ca2+ influx 3.NO is activated as secondary messanger. 4.NO released from endo cell. 5.Activates GC in sm 6.Activates PKG 7.Inc MLCP & vascular sm relaxation. |
How does length-tension relationship for smooth muscle differ from striated muscle? | It is a broader curve indicating it can form cross-bridges and generate tension over a wider range of lengths. **curves still look similar b/c the actin-myosin overlap principle is the same |
How does load-velocity relationship for smooth muscle differ from striated muscle? | it is less steep in smooth muscle. **Directly dependent on MLCK because MLC phosphorylation increases the rate of ATP hydrolysis and increases the velocity. |