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Yeast Identification
Testing procedures and important yeast characteristics
| Question | Answer |
|---|---|
| This organism has a "spaghetti and meatball" appearance microscopically. | Malassezia furfur |
| In what type of patients is Malassezia furfur capable of becoming systemic? | Newborns, anemics, or other patients that may be on a high lipid diet. |
| What is the leading cause of yeast infections in humans? | Candida albicans |
| True or False? Candida albicans in part of our normal flora. | True. May become problematic with changes in hormones, in the presence of antibiotics, or other changes. |
| Where do we see a problem with Candida albicans? | In the overweight population - in fat folds. In the immunocompromised |
| What is the mortality rate of immunocompromised patients with Candida albicans? | 65% - 95% |
| This disease is often one of the first disease to be seen in HIV patients and is caused by Candida species. | Candidiasis - more commonly known as thrush |
| This identification procedure uses dehydrated carbon in specific wells to pick up the pattern of different sugars used by yeast. | API 20C AUX |
| What are drawbacks of API 20C AUX? | Takes up to 72 hours Yeast like(polymorphic)molds could give false ID |
| This yeast ID method is a qualitative microtiter method. Is it more or less sensitive than API 20C AUX? | RapID Yeast Plus System - this is less sensitive but takes only hours for results. |
| What is a drawback of RapID Yeast Plus System? | Uncommon yeasts may be misidentified. |
| Oral Thrush, Vulvovaginitis, Skin & Nail infections, and Systemic infections are signs of which disease? | Candidiasis |
| What are the 6 species most commonly responsible for Candidiasis? | 1. Candida albicans 2. Candida glabrata 3. Candida parapsilosis 4. Candida tropicalis 5. Candida lusitaniae 6. Candida dublinensis |
| Which 2 Candida species are germ tube positive? How can they be distinguished from one another? | C. albicans & C. dublinensis Growth temp can be used to differentiate: C. albicans - 42°C C. dublinensis - 37°C |
| Which candida species can be difficult to treat due to its decreased susceptibility to azoles? | Candida glabrata |
| Which candida species is characterized by the presence of blastoconidia alone? | Candida glabrata |
| This candida species produces psuedohyphae and is dark blue on ChromAgar. | Candida tropicalis |
| Which candida species is commonly found on the hands of healthcare workers. | Candida parapsitosis |
| This yeast if commonly associated with bird droppings. | Cryptococcus neoformans |
| What structure does Cryptococcus neoformans have to increase it's virulence? What stain is used to detect this structure? | A capsule - seen in the presence of India Ink |
| C. neoformans is the leading cause of what disease in HIV patients? | Meningitis |
| What color does C. neoformans change to on birdseed agar? | It changes from white to brown |
| This organism is found on the eucalyptus leaves and is identical to C. neoformans. | Cryptococcus gattii |
| What structures are present microscopically for Trichosporon asahii? | Pseudohyphae, Arthroconidia, and Blastoconidia |
| Describe the colony appearance of Trichosporon asahii. What does this organism cause? | Dry, white, and farinose (velvety)and may have a cheese-light odor This organism can cause blood diseases and meningitis. |
Created by:
AshleySchutz
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