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Micro Practical 1
Microbiology Practical 1
| Question | Answer |
|---|---|
| Haemophilus influenzae Morphology, Media, Test | GN coccobacillus ONLY grows on CHOC Req. X and Y to grow |
| Haemophilus parainfluenzae Morphology, Media, Test | GN coccobacillus ONLY grows on CHOC Only req. V to grow |
| Haemophilus parahemolyticus Morphology, Media, Test | GN coccbacillus ONLY grows on CHOC Only req. V to grow, Hem+ |
| Haemophilus paraphrohiles Morphology, Media, Test | GN coccbacillus ONLY grow on CHOC Only req. V to grow |
| Staphylococcus aureus Morphology, Media | GPC(clusters) BAP=beta hem MSA=+Ferm =Pink |
| Staphylococcus epidermidis Morophology, Media | GPC(clusters) MSA=+Ferm=yellow Non Beta Hem |
| Tests for Staphylococcus epidermis | Coag - Susceptible to Novobiocin |
| Staphylococcus saprophyticus Morphology, Media | Non Beta Hem Coag - |
| Test used for Staphylococcus saprophyticus | Coag - Resistant to Novobiocin |
| Streptococcus pyogenes Morphology, Media | GPC (chains) beta Hem on BAP (large zone) Non beta Hem |
| Test for Streptococcus pyogenes Strep A | Susceptible to A disk (bacitracin) PYR + CAMP + |
| Streptococcus agalactiae Strep B Morphology, Media | GPC (chains) beta Hem on BAP small zone |
| Streptococcus pneumoniae Morphology, Media | GP (diplococci) *alpha hem on BAP* |
| Test for Streptococcus pneumoniae | Bile esculin - susceptible to P disk (optochin) Bile solubility + |
| Enterococcus faecalis Morphology, Media | GPC (chains)shorts gamma hem on BAP |
| Test for Enterococcus faecalis | Bile esculin + Salt tolerance + PYR + |
| Positive Bile esculin distinguishes which two organisms? | Enterococcus faecalis from Streptococcus pneumoniae |
| Streptococcus bovis Morphology, media | GPC (chains) gamma hem on BAP |
| Salt tolerance distinguish between which two organisms? | Positive = Enterococcus faecalis Negative = Streptococcus bovis |
| Viridans Streptococcus | GPC (chains) lots alpha Hem on BAP |
| P disk (optochin) test distinguish between which two organisms? | Resistance = Viridans Strep Susceptible = Streptococcus pneumoniae |
| Bile solubility test distinguishes which two organisms? | Positive = Streptocuccus pneumoniae Negative = Viridans Strep |
| A disk (bacitracin) test distinguishes between which two organisms? | Resistance = Streptococcus agalactiae Susceptible = Streptococcus pyogenes |
| Which organism doesn't go on BAP? | Neisseria gonorrhoeae |
| Neisseria gonorrhoeae Morphology, Media | Intercelluar diplococci ONLY grows on CHOC |
| Neisseria gonorrhoeae Test used? | CTA glucose, Red NET test |
| Neisseria lactamica Gram stain results? | GN diplococci |
| Neisseria lactamica grows on what type(s) of agar? | CHOC, MTM, and BAP |
| Which organism only grows on CHOC and BAP? | Microcuccus catarrhalis |
| Which organism is an asaccharolytic? | Microcuccus catarrhalis |
| What organism has negative results for CTA test and NET test? | Microcuccus catarrhalis |
| Escherichia coli gram stain result? | GNR |
| Which organism is a positve fermenter on MAC and beta hemolytic on BAP? | Escherichia coli |
| What is the IMViC for E.coli? | ++-- |
| What is the TSI result for E. coli? | A/A H2S- |
| What are the distinguishing characteristics for E. coli? | Flat colonies with green metallic colonies on EMB |
| How to distinguish E.coli(fermenter) from non fermenting E.coli? | Non fermenting E.coli does not ferment on MAC agar it appears clear and with the TSI the tube will show K/A |
| Enterobacter aerogenes IMViC ? | --++ |
| Is Enterobacter aerogenes a fermenter? | No |
| What is the TSI result for E.aerogenes? | A/A |
| Which amino acid(s) does E. aerogenes result(s) is/are positive? | Lysine and Ornithine |
| Which amino acid(s) does E. pneumoniae result(s) is/are positive? | Lysine |
| Is E. pneumoniae a fermenter? | Yes on MAC |
| How does Proteus mirabilis look with gram staining? | GNR |