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Micro Practical 1

Microbiology Practical 1

QuestionAnswer
Haemophilus influenzae Morphology, Media, Test GN coccobacillus ONLY grows on CHOC Req. X and Y to grow
Haemophilus parainfluenzae Morphology, Media, Test GN coccobacillus ONLY grows on CHOC Only req. V to grow
Haemophilus parahemolyticus Morphology, Media, Test GN coccbacillus ONLY grows on CHOC Only req. V to grow, Hem+
Haemophilus paraphrohiles Morphology, Media, Test GN coccbacillus ONLY grow on CHOC Only req. V to grow
Staphylococcus aureus Morphology, Media GPC(clusters) BAP=beta hem MSA=+Ferm =Pink
Staphylococcus epidermidis Morophology, Media GPC(clusters) MSA=+Ferm=yellow Non Beta Hem
Tests for Staphylococcus epidermis Coag - Susceptible to Novobiocin
Staphylococcus saprophyticus Morphology, Media Non Beta Hem Coag -
Test used for Staphylococcus saprophyticus Coag - Resistant to Novobiocin
Streptococcus pyogenes Morphology, Media GPC (chains) beta Hem on BAP (large zone) Non beta Hem
Test for Streptococcus pyogenes Strep A Susceptible to A disk (bacitracin) PYR + CAMP +
Streptococcus agalactiae Strep B Morphology, Media GPC (chains) beta Hem on BAP small zone
Streptococcus pneumoniae Morphology, Media GP (diplococci) *alpha hem on BAP*
Test for Streptococcus pneumoniae Bile esculin - susceptible to P disk (optochin) Bile solubility +
Enterococcus faecalis Morphology, Media GPC (chains)shorts gamma hem on BAP
Test for Enterococcus faecalis Bile esculin + Salt tolerance + PYR +
Positive Bile esculin distinguishes which two organisms? Enterococcus faecalis from Streptococcus pneumoniae
Streptococcus bovis Morphology, media GPC (chains) gamma hem on BAP
Salt tolerance distinguish between which two organisms? Positive = Enterococcus faecalis Negative = Streptococcus bovis
Viridans Streptococcus GPC (chains) lots alpha Hem on BAP
P disk (optochin) test distinguish between which two organisms? Resistance = Viridans Strep Susceptible = Streptococcus pneumoniae
Bile solubility test distinguishes which two organisms? Positive = Streptocuccus pneumoniae Negative = Viridans Strep
A disk (bacitracin) test distinguishes between which two organisms? Resistance = Streptococcus agalactiae Susceptible = Streptococcus pyogenes
Which organism doesn't go on BAP? Neisseria gonorrhoeae
Neisseria gonorrhoeae Morphology, Media Intercelluar diplococci ONLY grows on CHOC
Neisseria gonorrhoeae Test used? CTA glucose, Red NET test
Neisseria lactamica Gram stain results? GN diplococci
Neisseria lactamica grows on what type(s) of agar? CHOC, MTM, and BAP
Which organism only grows on CHOC and BAP? Microcuccus catarrhalis
Which organism is an asaccharolytic? Microcuccus catarrhalis
What organism has negative results for CTA test and NET test? Microcuccus catarrhalis
Escherichia coli gram stain result? GNR
Which organism is a positve fermenter on MAC and beta hemolytic on BAP? Escherichia coli
What is the IMViC for E.coli? ++--
What is the TSI result for E. coli? A/A H2S-
What are the distinguishing characteristics for E. coli? Flat colonies with green metallic colonies on EMB
How to distinguish E.coli(fermenter) from non fermenting E.coli? Non fermenting E.coli does not ferment on MAC agar it appears clear and with the TSI the tube will show K/A
Enterobacter aerogenes IMViC ? --++
Is Enterobacter aerogenes a fermenter? No
What is the TSI result for E.aerogenes? A/A
Which amino acid(s) does E. aerogenes result(s) is/are positive? Lysine and Ornithine
Which amino acid(s) does E. pneumoniae result(s) is/are positive? Lysine
Is E. pneumoniae a fermenter? Yes on MAC
How does Proteus mirabilis look with gram staining? GNR
Created by: MrsHo