Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Electrophoresis san.
explaining and the different types of electrophoresis
| Question | Answer |
|---|---|
| Define electrophoresis | Is the separation of molecules according to differences in their electric charge when they are placed in an electric field |
| particles that are negatively charged move toward the: | anode |
| particles that are positively charged move toward the: | cathod |
| define immunoelectrophoresis | electrophoresis of serum or urine followed by diffusion |
| what is diffusion? | protein fractions are allowed to act as antigens and interact with corresponding antibodies |
| what are the 5 components according to electrophoretic motility? | albumin, alpha1, alpha2, beta and gamma |
| what is monoclonal? | gammopathies are single clones of plasma cells that produce elevated levels of a single class of immunoglobulin |
| what is polyclonal? | gammopathies are caused by secondary conditions such as liver disease, collagen disorders, rheumatoid arthritis and chronic infection |
| what is immunoelectrophoresis used for? | for the detection of myelomas, Waldenstrom's macroglobulinemia, malignant lymphomas and other lymphoproliferative disorders and immunodeficiencies |
| What are the two stages of immunofixation electrophoresis? | electrophoretic separation and immunoprecipitation (the paper is full of gel) |
| immunofixation electrophoresis specimens may include the following | serum, urine,cerebrospinal fluid or other body fluids |
| immunoelectrophoresis vs immunofixation electrophoresis | IEP is less sensitive than IFE IFE can separate any monoclonal band separated on SPE IEP more difficult to interpret than IFE IEP is a longer test due to incubation time (18 hr) IEP is relative less expensive and less labor-intensive than IFE |
| what is Heterogeneous immunoassays? | it involves a solid phase(microwell, bead) and require washing steps to remove unbound antigens or antibodies( can be competitive or noncompetitive) |
| what is Homogeneous immunoassays? | It consists of only a liquid phase and do not require washing steps (methods are faster and easier to automate, can have a competitive format) |
| 4 categories for indicator labels are? | radioactive isotopes, chemical light, fluorochromes, and enzymes |
| What is competitve immunoassay? | a test in which labeled and non-labeled antigen competes for the same spot on an antibody. |
| How competitve immunoassay works? | the amount of label is inversely propportional to the amount of analyte ( antigen or antibody) measured |
| what is sandwich immunoassay? | is a non-competitive assay in which the amount of label is directly proportional to the analyte (AG or AB) |
| What is enzyme immunoassay? (EIA) | uses a nonisotope label, are designed to detect ag or ab by producing an enzyme color change |
| What are the 4 common enzyme labels (EIA) | horseradish peroxidase, alkaline phosphatase, glucose-6-phosphatase dehydorgenase and beta-galactosidase |
| what test is the number one for lupus? | ANA |
| what is chemiluminescence? | refers to light emission produced during a chemical reaction and is used extensively in automated immunoassays |
| indirect immunofluorescent assay is: | it is used extensively in the detection of autoantibodies and antibodies to tissues and cellular antigens such as ANA |
| direct immunofluorescent assay is: | Fluorescein-conjugated antibodies bound to the fluorochrome FITC are used to visualize many bacteria in direct specimens |
Created by:
lesliederby
Popular Laboratory Science sets