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Electrophoresis san.

explaining and the different types of electrophoresis

QuestionAnswer
Define electrophoresis Is the separation of molecules according to differences in their electric charge when they are placed in an electric field
particles that are negatively charged move toward the: anode
particles that are positively charged move toward the: cathod
define immunoelectrophoresis electrophoresis of serum or urine followed by diffusion
what is diffusion? protein fractions are allowed to act as antigens and interact with corresponding antibodies
what are the 5 components according to electrophoretic motility? albumin, alpha1, alpha2, beta and gamma
what is monoclonal? gammopathies are single clones of plasma cells that produce elevated levels of a single class of immunoglobulin
what is polyclonal? gammopathies are caused by secondary conditions such as liver disease, collagen disorders, rheumatoid arthritis and chronic infection
what is immunoelectrophoresis used for? for the detection of myelomas, Waldenstrom's macroglobulinemia, malignant lymphomas and other lymphoproliferative disorders and immunodeficiencies
What are the two stages of immunofixation electrophoresis? electrophoretic separation and immunoprecipitation (the paper is full of gel)
immunofixation electrophoresis specimens may include the following serum, urine,cerebrospinal fluid or other body fluids
immunoelectrophoresis vs immunofixation electrophoresis IEP is less sensitive than IFE IFE can separate any monoclonal band separated on SPE IEP more difficult to interpret than IFE IEP is a longer test due to incubation time (18 hr) IEP is relative less expensive and less labor-intensive than IFE
what is Heterogeneous immunoassays? it involves a solid phase(microwell, bead) and require washing steps to remove unbound antigens or antibodies( can be competitive or noncompetitive)
what is Homogeneous immunoassays? It consists of only a liquid phase and do not require washing steps (methods are faster and easier to automate, can have a competitive format)
4 categories for indicator labels are? radioactive isotopes, chemical light, fluorochromes, and enzymes
What is competitve immunoassay? a test in which labeled and non-labeled antigen competes for the same spot on an antibody.
How competitve immunoassay works? the amount of label is inversely propportional to the amount of analyte ( antigen or antibody) measured
what is sandwich immunoassay? is a non-competitive assay in which the amount of label is directly proportional to the analyte (AG or AB)
What is enzyme immunoassay? (EIA) uses a nonisotope label, are designed to detect ag or ab by producing an enzyme color change
What are the 4 common enzyme labels (EIA) horseradish peroxidase, alkaline phosphatase, glucose-6-phosphatase dehydorgenase and beta-galactosidase
what test is the number one for lupus? ANA
what is chemiluminescence? refers to light emission produced during a chemical reaction and is used extensively in automated immunoassays
indirect immunofluorescent assay is: it is used extensively in the detection of autoantibodies and antibodies to tissues and cellular antigens such as ANA
direct immunofluorescent assay is: Fluorescein-conjugated antibodies bound to the fluorochrome FITC are used to visualize many bacteria in direct specimens
Created by: lesliederby