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Reagent PadPrincipleFalse +False -Color ChangeNormal ValueDisease
Leukocyte Granulocytic leukocyte esterase-azo coupling Menstrual Contamination, pigments Increased specific gravity, glucose, protein Beige to violet Neg Inflammation, bacterial infection
Nitrite Griess test, Diazotization reaction, azo dye formation Medications, pigments Ascorbic acid White to pink Neg UTI, pyelonephritis
Urobilinogen Ehrlich's reaction-azocoupling Ehrlich reactive substances Nitrates, formalin Peach to pink 0.1-1.0 Ehrlich unit/dL Liver disease
Protein Protein error of indicator. Tetrabromphenol blue X ray contrast media protein other than albumin Yellow to blue/green Neg Kidney disease
pH Double indicator Methyl red & bromthymol blue Bacterial breakdown of uria Bacterial breakdown of glucose Orange to green to blue 4.5-8.0 Acidic-diabetes Alkaline-kidney
Blood Pseudoperoxidase activity of heme portion of Hgb Menstrual Contamination Ascorbic acid, high specific gravity Yellow to dark green Neg Kidney disease
Specific Gravity PKa change of polyelectrolyte, Ions release H+ Protein 100-500 mg/dl High glucose, urea or pH >6.5 Blue/green to yellow/green 1.002-1.035 Constant 1.010 = isosthenuria
Ketone Sodium nitroprusside reacts with acetoacetic acid Drugs, pigments Improper storage, bacterial breakdown of acetoecacetic acid Beige to pink/purple Neg Diabetes
Bilirubin Coupling reaction w/ diazonium salt Pigments Ascorbic acid Tan to Beige/pink Neg Liver disease
Glucose Double enzyme reaction (glucose oxidase) Bleach Ascorbic acid Blue/green to brown Neg Diabetes
Created by: thesmokinggun
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