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Adv Micro Fun

QuestionAnswer
Microsporum Audouini: Colony morphology front and reverse Surface flat, downy to silky, grayish or tanish white. Reverse is light salmon with reddish-brown center.
Microsporum Audouini: Microscopic morphology Septate hyphae with terminal chlamydoconidia that is often pointed on the end. This species is usually devoid of conidia.
Microsporum Audouini: Confirmatory Tests Unable to grow on Polished Rice grains while other Microsporum species grow well.
Microsporum Canis: Colony morphology Front and reverse Surface whitish, coarsely fluffy, with yellow pigment at periphery; closely spaced radial grooves. Reverse is deep yellow and turns brownish yellow with age.
Microsporum Canis: Microscopic morphology Septate hyphae with numerous macroconidia, which are long, spindle shaped, rough, thick walled, and taper to knoblike ends (prominent snout). Usually more than six compartments.
Microsporum Gypseum: Colony morphology Front and reverse Surface flat and spreading, powdery to granular, buff at first, then tan to cinnamon brown. Reverse may be yellow, orange tan, brownish red.
Microsporum Gypseum: Microscopic Morphology Septate hyphae. Macroconidia in enormous numbers, rough, relatively thin walled with no more than six compartments. The ends are rounded. Microconidia, club shaped, along the hyphae.
Trichophyton Metagrophytes: Colony morphology Front and reverse Varies greatly; surface may be buff and powdery or white and downy, white with concentric and radial folds. Reverse is usually brownish.
Trichophyton Metagrophytes: Microscopic Morphology Septate hyphae. The arrangements of microconidia on the hyphae are used for identification. Microconidia are globose, and clustered on branched conidiophores. Many coiled spirals are often seen.
Trichophyton Metagrophytes: Confirmatory Test Urease: +, Hair penetration test: +
Trichophyton Rubrum: Colony morphology Front and reverse Surface granular to fluffy, white. Reverse is deep red. The best production of pigment is best seen on cornmeal agar.
Trichophyton Rubrum: Microscopic morphology Septate hyphae with lateral, teardrop-shaped microconidia. Macroconidia are the same for all Trichophyton sp. Microconidia form directly on hyphae in thurses.
Trichophyton Rubrum: Confirmatory Test Urease =, hair penetration test =
Trichophyton Tonsurans: Colony morphology Front and reverse Highly variable. Surface may be white, grayish, yellow, rose, or brownish. Surface usually suedelike, with many radial or concentric folds. Reverse is usually reddish brown, sometimes yellow or colorless.
Trichophyton Tonsurans: Microscopic morphology Septate hyphae with many variably shaped microconidia all along the hyphae or on short conidiophores. Microconidia are usually teardrop or club shaped, but may be elongate or enlarge to round “balloon” forms.
Epidermophyton Floccosum: Colony morphology Surface brownish yellow to olive gray or khaki; at first lumpy and sparse, then folded in center and grooved radially, becoming velvety.
Epidermophyton Floccosum: Microscopic Morphology Septate hyphae; no microconidia. Macroconidia, seen best in young cultures, are smooth, both thin and thicker walled, club shaped with rounded ends, contain two to six cells,and are found singly or in characteristic clusters. Chlamydoconidia present
Sporothrix Schenckii: Colony morphology A low, flat, smooth colony develops that is usually dirty white to grey-brown turning black (front and reverse) with age - becomes tough, wrinkled and folded with age (never develops floccose aerial mycelia). Looks like a wet or oily mouse's coat.
Sporothrix Schenckii: Microscopic Morphology Thin, delicate hyphae, septate and branching, with slender, tapering conidiophores arising at right angles to the hyphae. There are small pear-shaped, almost round, conidia on delicate threadlike denticles, forming a rosettelike cluster.
Sporothrix Schenckii: Dimorphism Subculture to BHI with Blood and incubate at 37 C - yeast colonies grow. Make a wet prep - small elongate oval the round Yeast "cigar bodies" with single and multiple buds, which may have, short necks.
Cladosporium spp.: Microscopic Morphology Septate hyphae, dark. Conidiation is of the Cladosporium type only.
Fonsecaea Pedrosoi: Microscopic Morphology Hyphae, septate, branched. Three types of conidial formation may be seen: Cladosporium, Rhinocladiella (most common), and Phialophora types.
Phialophora spp.: Microscopic Morphology Dark hyphae, branched, septate, with vase-shaped Phialophora-type conidiophores. At the end of the conidiophores are clusters of conidia resembling flowers in a vase.
Pseudallescheria Boydii(Telomorph)/Scedosporium Apiospermum: Colony Morpology Spreading, white, cottony, later turning gray or brown. Reverse is at first white, becoming gray or black.
Pseudallescheria Boydii(Telomorph)/Scedosporium Apiospermum: Microscopic Morphology Septate hyphae with simple long or short conidiophores bearing conidia singly or in small groups. The conidia are unicellular, and oval. In the sexual stage, cleistothecia are formed and release elliptic ascospores when ruptured.
Acremonium spp.: Colony morphology Compact,folded,and feltlike, then becomes overgrown with loose cottony hyphae. May be white, gray, or rose in color.
Acremonium spp.: Microscopic Morphology Extremely delicate, septate hyphae, with unbranched, tapering conidiophores. Conidia are oblong, usually one celled but occasionally two celled.
Created by: Roivas