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Biotech Ch3c

Biotech Essentials

QuestionAnswer
A gel-like substance made from agar that provides a solid surface for growing bacterial colonies on petri dishes Solid Agar Media
nutrient-rich growth medium commonly used to culture bacteria, especially *E. coli*, for molecular biology applications LB / Luria-Bertani Broth
technique using antibiotics in media to grow only bacteria with desired genetic modifications or plasmids Antibiotic Selection
method to test bacterial susceptibility to antibiotics by measuring the clear zone around antibiotic-soaked disks on an agar plate Kirby-Bauer/Disk Diffusion Test
handheld tool with a wire or plastic loop used to transfer bacteria between locations Inoculation Loop
Large-scale vessels that provide controlled conditions for growing massive quantities of microorganisms or cells Bioreactors/Fermenters
Procedures to prevent contamination during experiments, including sterilizing equipment and minimizing air exposure Aseptic Technique
An enclosed workspace with filtered air flow that creates a sterile environment for working with cells and microorganisms Tissue Culture Hood/Bio Safety Hood
Growing cells under controlled laboratory conditions in nutrient media for research and product development Cell Culture
A visible cluster of genetically identical bacterial cells grown from a single cell on solid media Colony
A unit that estimates the number of viable bacteria in a sample based on colony count CFU (colony forming unit)
A measurement of light blocked by a bacterial culture, indicating cell concentration and used to monitor growth OD (Optical Density)
Created by: MCHSBiotech
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