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Biotech Ch 2c
Biotech Essentials
| Term | Definition |
|---|---|
| Metric Prefixes | used to deal with very large or very small quantities |
| Gram | base metric unit of mass |
| liter | base metric unit of volume |
| meter | base metric unit of length |
| milli | thousandth (m) |
| micro | millionth (µ) |
| nano | billionth (n) |
| solution | a liquid that has a solute dissolved in it |
| solute | the dissolved matter in a solution |
| solvent | a liquid that can dissolve other substances |
| m/v % equation | mass per volume percent solutions |
| v/v % equation | volume per volume percent solutions |
| C1V1 = C2V2 | concentration 1 x volume 1 = concentration 2 x volume 2 |
| stock solution | concentrated reagent or chemical solution that is diluted to a lower working concentration when needed for experiments or assay |
| buffer | solution that resists changes in pH to maintain a stable chemical environment for cellular processes, enzymes, and proteins |
| TAE | Tris-Acetate-EDTA buffer solution used in agarose gel electrophoresis |
| TBE | Tris-Borate-EDTA buffer solution used primarily for nucleic acid gel electrophoresis. |
| PBS | Phosphate-buffered saline commonly used in ELISA immunoassays |