Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Biotechnology
YGK These Techniques in Biotechnology
| Question | Answer |
|---|---|
| Technique used to amplify small segments of DNA | PCR (polymerase chain reaction) |
| Inventor of PCR in 1983 | Kary Mullis |
| Instrument used for PCR cycles | Thermal cycler |
| Three steps of PCR | Denaturation, annealing, and extension |
| Heat-stable DNA polymerase used in PCR | Taq polymerase |
| Bacterial source of Taq polymerase | Thermus aquaticus |
| PCR variant used to measure RNA by converting it to cDNA | RT-PCR (reverse transcription PCR) |
| Technique to separate molecules by size and charge | Gel electrophoresis |
| Gels used as matrices in electrophoresis | Agarose or polyacrylamide |
| Buffers used to maintain pH and conduction in electrophoresis | TAE and TBE |
| Stain used to visualize nucleic acids in a gel | Ethidium bromide |
| Stain used to visualize proteins in a gel | Coomassie brilliant blue |
| Standard set of molecules with known sizes | Ladder |
| Electrophoresis variant using SDS to give proteins a negative charge | SDS-PAGE |
| Process of determining the exact order of nucleotides | DNA sequencing |
| Sequencing method using chain-terminating ddNTPs | Sanger sequencing |
| Sequencing method that reassembles overlapping random fragments | Shotgun sequencing |
| UK-based company using nanopore sequencing | Oxford Nanopore Technologies |
| Blotting technique used to detect RNA | Northern blot |
| Blotting technique used to detect proteins | Western blot |
| Blotting technique used to detect DNA | Southern blot |
| Inventor of the Southern blot | Edwin Southern |
| Membranes used for protein blotting | Nitrocellulose or PVDF |
| Antibodies used in Western blotting | Primary and secondary antibodies |
| Assay based on antigen-antibody interaction and color change | ELISA |
| Most popular type of ELISA | Sandwich ELISA |
| Genome-editing technology derived from bacterial immune systems | CRISPR/Cas9 |
| Winners of the 2020 Nobel Prize for CRISPR discovery | Jennifer Doudna and Emmanuelle Charpentier |
| The two key components of CRISPR/Cas9 | Cas9 enzyme and guide RNA (gRNA) |
| Repair mechanism that joins DNA ends and often introduces mutations | Non-homologous end joining (NHEJ) |
| Repair mechanism that uses a template to insert new DNA sequences | Homology-directed repair (HDR) |
| Gene targeted by He Jiankui in 2018 for HIV resistance | CCR5 |
| Analyzing cell characteristics in a stream via laser | Flow cytometry |
| Light scatter used to determine cell size in flow cytometry | Forward-scatter (FSC) |
| Light scatter used to determine cell granularity | Side-scatter (SSC) |
| Techniques ensuring cells pass through a laser in single file | Hydrodynamic or acoustic focusing |
| Flow cytometry used to sort cells based on fluorescence | FACS (Fluorescence-activated cell sorting) |
| Process of creating multiple copies of a specific DNA segment | Molecular cloning |
| Circular DNA molecule often used as a vector in cloning | Plasmid |
| Enzymes used to cut DNA at specific sequences | Restriction enzymes |
| Enzyme that joins DNA fragments and vectors together | DNA ligase |
| Introduction of a recombinant vector into a host cell | Transformation |
| Feature added to vectors to ensure only transformed cells grow | Antibacterial resistance gene |
Created by:
divyap
Popular Quiz Bowl sets