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CHEM DETECTION METH

MLT 110: CLINICAL CHEMISTRY LECTURE

QuestionAnswer
In the Urinalysis department, what form of photometry is used to measure color changes in biochemical tests? Refractometry
Why would the Hematology department utilize the Coulter Principle? it automates cell counting by measuring the number of pulses made when the RBC interrupts an established electric pathway, and it measures cell volume based on the magnitude
What is the difference between Photometry and Spectrophotometry? Photometry does not require a certain wavelength to measure light intensity, and the substance does not require coloring to be viewed
What is the meaning of an Endpoint detection method? Coagulation occurs before the measurement is taken
Describe Electrical Impedance the number of RBCs (pulses) that interrupt an established electrical current, paired with the magnitude of the pulse (equal to cell volume)
What is the relationship between absorbance and concentration? The more light that is absorbed, the higher concentration of the substance
A Photodetector converts (blank) energy into (blank) energy light; electrical
Monochromator isolates wavelengths
What is the relationship between light absorbance (A) and percent transmission (%T)? greater absorbance=less transmittance of light
According to Beer's Law, a negative slope would indicate an inverse proportional relationship between transmission and substance concentration. the transmittance lessens as concentration grows
According to Beer's Law, absorbance's proportional relation to a substance's concentration would be indicative of what type of slope? positive slope
Why do we need to use a reagent blank? To set the instrument at Zero; negates all light that may be accidentally absorbed by reagent's chemicals
What Clinical Chemistry detection method is used on divalent or trivalent ions in trace evaluations? Atomic Absorption Spectrophotmetry
What occurs in Atomic Absorption Spectrophotometry the method quantifies the absorption of radiation at a specific wavelength via the generation of free atoms
Which form of photometry is used in clinical chem, hematology, and urinalysis to determine the degree of cloudiness within a solution? turbidometry
In the Coagulation department, what type of detection method would be used to indicate clot formation? Optical endpoint
What affect does the presence of fibrin have on optical endpoint detection? The formation of active fibrin from inactive fibrinogen would decrease light transmission
Which form of photometry scatters light at a right angle? Nephelometry
What does electrical endpoint detection detect? change in electrical conductivity due to coagulation
In Hematology, how is Flow Cytometry utilized? it categorizes cells based on size, how much light they scatter, and how they bind to fluorescent dye
Electrophoresis is used to separate what kind of substance? mixture of proteins or nucleic acids
Fluorometry is utilized in the lab to measure emission of light by a substance
Describe Chemiluminescence and how it differs from Fluorometry chemical reaction causes rapid uptick in light intensity that gradually decays like a glowstick; it does not require radiation excitement because it is an oxidation reaction
What Clinical Chemistry detection method is used to analyze the electric potential between two electrodes Potentiometry/Electrochemistry
What are four types of electrodes that can be assessed using Electrochemistry? - Ion selective - pH - gas sensing - enzyme
In Electrophoresis, what kind of dye would track the progression loading/tracking
Marker dyes would bind to (blank) to show the aftermath of UV light causing a material to glow DNA segments
Describe Chromatography separation of mixtures based on their different physical interactions
Which Clinical Chemistry detection methods would be useful for urinalysis drug testing? Gas Chromatography/Mass Spectrophotometry
Describe Mass Spectrometry identification of substances from gas chromatography
Created by: innazukaa
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