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PRACTICAL 1

LAB #1, #4-6

QuestionAnswer
What substance do we use to wipe down benches at the end of lab? 10% bleach solution
Which sink is NOT used for handwashing? Room 346 (back sink)
What is the back sink in 346 used for? Pipette washing and urine disposal
What safety items can be found on the radiators? first aid kit, biohazard spill kit
What safety items can be found on the vents? MSDS Manual (front vent), first aid kits, Laboratory SOPs
Where is the Fire Safety Blanket? above the front fire extinguisher
Where is the Flammable Cabinet located? Room 346, under the TV
Which sink is used for handwashing Front sink
Where are the Personal Exposure Plans located? 1) front, near the chalkboard 2) Back by the eyewash station
When would Graduated Cylinders be used? when a high degree of accuracy is not essential
Most graduated cylinders are calibrated as to-deliver
TD means that the desired volume is delivered to the apparatus by gravity, and the remaining value is meant to remain in the pipette
TC means to contain; the desired volume is delivered to the apparatus without the exact amount being calibrated to be delivered. then, meant to rinse out the pipette with diluting fluid
What is the calibration of graduated cylinders? TD
What is the function of a graduated cylinder? prepping for lab reagents
What would the curve of meniscus depend on? surface tension of the liquid (molecules are more attracted to each other - convex) and adhesion to the wall of the container (strongly attracted to the container - concave)
What is the most common form of beaker used in the lab? Griffin low form
Beakers are used for general mixing and reagent prep
Erlenmeyer flasks are used for preparing reagents and for titration procedures
Volumetric flasks are used in preparation of specific volumes of reagents or lab solutions
Why are volumetric flasks repeatedly mixed during dilutions? To make the mixture homogenous before they are made up for volume
What is the calibration of volumetric flasks? To contain
What is a convex meniscus? Produced when molecules are more attracted to each other than the container
What is a concave meniscus? Produced when molecules are more attracted to the container than the molecules themselves
When measuring unknown bodily fluids, what type of pipette would we use and why? Volumetric; because it produces accurate measurements of volumes that are 1ml or more
If we need to measure a reagent, what type of pipette would we use and why? Graduated; because great accuracy is not critical
What is the difference between a Mohr pipette and a Serological pipette? MOHR- calibration mark does not go to the tip SEROLOGICAL- calibration mark goes to the tip, so it needs to be blown out mechanically
Why was a Serological pipette designed for serology? Relative values are sought after
Which pipette is the most accurate? Volumetric
Which pipette is the most precise? Automatic
What tube is used for blood cultures? Gray top vacutainer tube with sodium fluoride
What tube is used for urinalysis? Tiger tube
What tube is used for urine cultures? Gray top tube with boric acid to keep microbes alive and stabilize the pH
When would a long swab with media be used? To gather microbes from open wounds, vagina or throat for in-vitro diagnostic testing only
What equipment would be needed for occult fecal screens? Hemoccult
A blood culture collection kit is used to detect bacteria in the blood
Ova and parasite in-vitro diagnostic testing is achieved using what equipment? Parapak
Molarity = mols of solute divided by volume (L)
Mols = given mass divided by molar mass
Created by: innazukaa
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