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CHAPTER 4
BASIC MICROSCOPE OPS
| Question | Answer |
|---|---|
| What microscope is most used within the lab? | Brightfield (compound light) |
| What microscope components are a part of the light system | condenser, iris diaphragm, field diaphragm |
| What is the function of a condenser? | direct and focus light to ensure the image field is evenly lit |
| What is the function of the aperture iris diaphragm? | controls the amount of light |
| What is the typical magnification for the eyepiece? | 10x |
| What does Inter-pupillary distance do? | adjust the distance between oculars |
| What is focal distance? | working distance from the objective to the item |
| Low power objectives have a focal length of | 16mm |
| High, dry power objectives have a focal length of | 4mm |
| Oil immersion objectives have a focal length of | 1.8mm |
| When doing morphological exams of blood or microbes, what would the objective magnification be? | 100x |
| A focal distance of 4mm would be used to study what? | urine sediments and histology |
| A focal distance of 16mm would be used for | scanning |
| What is the magnification of "scanning"? | 4x |
| What is the magnification of high, dry objective? | 40x |
| Within the Focus System, when the light come down from the oculars, where does it go to? | Body tube |
| What does the body tube do? | move up and down to focus in objects |
| What can total magnification be defined as? | Objective mag. x Ocular mag |
| What is resolution | how small and close an object can be while also being recognizable and differential from the individual objects around it |
| NA stands for | numerical aperture |
| What is Numerical Aperture (NA)? | a measure of the light's ability to gather within a microscope |
| How does NA directly related to resolution? | As NA increases, so does the resolution |
| What happens if we were to decrease the amount of light passing through the lens | Creates an inverse relationship with NA because it decreases the NA |
| What is parafocal | When an image is in focus, rotating objective lens only requires minimal fine adjustments (the original image is not lost) |
| When using a Brightfield Microscope, how would the background and elements look like? | The elements appear slightly dark, while the background is very light grey |
| When counting platelets or identifying casts/cells in what microscope would be used and why? | Phase contrast; unstained structures are differentiated by light intensity |
| Darkfield microscopes have light that is (______) to travel in (__________________) | forced; oblique ajges |
| Which microscope would be used to detect spirochetes? | Darkfield |
| DIC stands for | Differential Interference Contrast |
| DIC microscopes produces | 3-D imaging |
| What does a polarizing light microscope do? | filters light by allowing specific wavelengths through |
| Identifying crystals is done by using ___________ ________ microscopes | polarizing light |
| Fluorescence Microscope is used during what kind of test? | Fluorescent Antibody tests |
| What microscope increases resolution and magnification | Electron |
| What are Electron Microscopes used for? | In vitro study of cells and tissue |
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