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Properties of water
Uni of Notts, core skills in biochemistry, first year
| Term | Definition |
|---|---|
| How the liquid temperature range of water supports life | Water is a liquid at a range of temperatures where many vital chemical reactions occur at a reasonable rate |
| How water reacts with hydrophilic solutes | The positive/negative poles of water are attracted to oppositely charged solutes forming a hydration sphere around them |
| How water reacts with hydrophobic substances | It will first exclude the substance by forming an ordered cage around it but this is too low entropy so the substance then aggregates together in the water |
| How DNA (& similar molecules) interact with water | The phospho-diester backbone is hydrophilic allowing it to interact with water but nitrogenous bases are hydrophobic. This protects the bases from nucleophilic attack which causes DNA damage |
| How auto-ionisation of water occurs | Molecules constantly move (vibrate) due to extension & shortening of bonds. If one stretches in the vicinity of another, this can lead to the protonation of one water (i.e., H3O+ & OH-) |
| Isoelectric point (pI) | pH at which an amino acid (& by extension protein) contains a net neutral charge of protonated & deprotonated groups across its structure, measured in pI |
| Isoelectric focusing | Method of separating proteins by running them through gels of different pHs. When they reach their isoelectric point they'll stop being attracted by the electrode & stop moving |
Created by:
Beech47
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