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Protozoans
| Question | Answer |
|---|---|
| Single-celled (unicellular), eukaryotic organisms | Protozoans |
| Protozoan nucleus contains: (2) | Karyosome, Peripheral Chromatin |
| Protozoan ingest through a small mouth called: | cytostome |
| Protozoan Reproduction: | Asexual - Binary Fission |
| AMOEBAS All have cystic stage except for | Entamoeba gingivalis |
| AMOEBAS All are commensal except for | Entamoeba histolytica |
| Amoeba organism with peripheral chromatin in both trophozoite and cyst and chromatoidal bar in cyst only: | Entamoeba (true amoeba) |
| Amoeba organism with no peripheral chromatin nor chromatoidal bodies | Endolimax or lodamoeba (other amoeba) |
| Amoeba CYST or TROPH: Motile (pseudopod) | TROPHOZOITE |
| Amoeba CYST or TROPH: Feeding Stage | TROPHOZOITE |
| Amoeba CYST or TROPH: Excystation (occurs in the small intestine) | TROPHOZOITE |
| Amoeba CYST or TROPH: Encystation (occurs in the large intestine) | CYST |
| Amoeba CYST or TROPH: Destroyed by lodine stain | TROPHOZOITE |
| Laboratory Diagnosis of Amoeba (3) | Saline wet preparations, iodine wet preparations, and permanent stains |
| Show motility of amebic trophozoites, internal cytoplasmic and nuclear structures | Saline wet preparation |
| Confirm parasite identification | Permanent smear |
| Obtain organism size using: | Ocular micrometer |
| Diagnosis of ____________ is important because this finding suggests ingestion of contaminated food or drink. | Nonpathogenic amoeba |
| CA: Intestinal amebiasis, amebic colitis, amebic dysentery, extraintestinal amebiasis | Entamoeba histolytica |
| Progressive, directional movement | Entamoeba histolytica |
| Endoplasm contains ingested RBC but no bacteria / cell debris (ground glass) | Entamoeba histolytica |
| Cyst: Coffin-shaped / rod-shaped / sausage-shape chromatoidal bar | Entamoeba histolytica |
| Entamoeba histolytica cyst passed out into the environment in human feces may survive for up to: | 1 month |
| Flask-Shaped ulceration | Entamoeba histolytica |
| Tx: E. histolytica | Metronidazole (Flagyl) |
| Infective (quadrinucleated) cyst of E. histolytica is resistant to: | Routine chlorination |
| Method of choice for discriminating between E. histolytica and non-pathogenic amoeba E. dispar: | Molecular analysis by polymerase chain reaction (PCR) |
| Phoretic Vectors of E. hitsolytica: | Cockroach and Flies |
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