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Micro Anaerobes

Micro Anerobes

QuestionAnswer
Only NON-MOTILE Clostridium Species. perfringens
Gram positive bacillus that are BOXCAR shaped. C. perfringens
When plated with S. aureus, this species performs a REVERSE CAMP (blunt ends)on SBA. C. perfringens
This species causes GANGRENE. C. perfringens
This species has terminal spores that look like DRUMSTICKS. C. tentani
This species causes TETANUS or "LOCKJAW" C. tentani
What two toxins does C. tetani have? Tetanolysis and Tetanoplasmin
This species has endospores that look like TENNIS RACKETS. C. botulinum
Jarred jellies and processed meats can cause this toxin Botulism
A and B enterotoxins are found in this species. C. difficile
On CCFA media, these colonies turn yellow. C. difficile
This species has a SAFETYPIN appearence. Bacteroides fragilis
Spindle shaped morphology that is common in respiratory and GI tract. Fusobacterium Sp.
Causes lung and dental infections Prevotella melaninogenica
Grows black pigmented colonies Prevotella melaninogenica
Causes purple pigmented agar Porphyromonas
"Anaerobic Staph" Peptococcus
"Anaerobic Strep" Peptostreptococcus
Rapid ID ANA remel, API 20 1 biomerieux,and BBL crystal ANA ID are examples of... Biochemical System testing
Darkfield and Westernblot Are other ways to ID anaerobes
tightly coiled Helix that have spiral rotations Leptospira interrogans
Reservoir of cat, dog and rats Leptospira interrogans
Causes LYME disease Borriela burgdorferi
What species of tick causes Lyme disease Ixodes
Causes the "BULLS EYE RASH" Borriela burgdorferi
Pediculus humanus (human louse) Borriela recurrentis
Soft tick species that causes B. hermsii, B. parkeri, B. turicatae Ornithodoros
This species cause SYPHILIS Treponema pallidum
Latent syphilis causes reappearence of lesions
Tetiary syphilis causes Tissue damage
Congenital syphilis causes baby infections
Hutchinsons teeth Treponema pallidum
"ROCKY MOUNTAIN SPOTTED FEVER" Rickettsiae
This species must be cultured on "non artifical media" Rickettsiae
The species first appears with a RASH on ANKLES and WRISTS Rickettsiae
Catch from Cat and Rat fleas Typhus
What test can be used to determine typhus Weil Felix test
STD, and cause eye infections Chlamydia trachomatis
Linked to coronary heart disease and is a human infection only Chlymydia pneumoniae
Causes "PARROT FEVER" Clamydia psittaci
Tissue culture McCoy cells
Detects outer membrane DFA-Direct Florescent Antibodies
Positive PPD Tuberculosis mycobacterium
PPD stands for Purified Protein Derivative
Nitrate and Catalase Positive TB
Found in AIDS patients and is similar to TB Mycobacterium avium (MAC)
On photochromogen, this species is non pigmented in dark and yellow in light Mycobacterium kansasii
Causes skin and soft tissue disease Mycobacterium fortuitum
Hansens Disease Mycobacterium leprae
LEPROSY is caused by Mycobacterium laprae
Tuberculoid skin lesions
Lepromatous cartlidge distruction
Fish disease Mycobacterium marinum
Causes cervical lymphadentitis Mycobacterium scrofulaceum
Smooth buttery colonies Mycobacterium scroflaceum
Found in water taps and soil Mycobacterium gordonae
grows in 10-14 days and has smooth yellow/orange colonies Mycobacterium gordonae
High risk in crowded areas (shelters, schools, etc.) Mycoplasma pneumoniae
WALKING PNEUMONIAE mycoplasma pneumoniae
"FRIED EGG" appearence on PPLO agar Mycoplasma pneumoniae
Genital tract ulcers mycoplasma hominis
STD of "MALES" Ureaplasma urealyticum
Detected by Rapid mycoplashea test Uraplasma urealyticum
Ureaplasma splits urea
Kills directly bactericidal
Prevents growth bacteriostatic
Artifical Synthetic
Chemically altered semi-synthetic
Antibiotic killing normal flora turns infection into... Super Infection
Enzymes for synthesis peptidoglycan
Prevents cell wall synthesis beta lactam
Used on grm positive and gram negative cocci Penicillin
Good for gram positive, modest against gram negative first generation cephalosorins
better action again gram negative b/c stable against beta lactamases second generation cephalosporins
less activity against gram positives but better against enterics third generation cephalosporins
acts against aerobic gram negative rods (p. aeruginosa) Monobactam
Broad spectrum of any microbial agaent Carbapenem
Combination with this drug can be synergistic beta lactem
Works only against Gram positive organisms Vancomycin
These are toxic when used as topical antibiotics Bacitracin and cycloserine
What commonly used topical antibiotic has Bacitracin Neospren
alters permeability and osmotic integrity Polymyxins
Binds IRREVERSIBLY to 30S ribosomal unit Aminoglycosides
Binds REVERSIBLY to 30S ribosomal unit Tetracyclines
IV antibiotics must be administered in the hospital due to,,, Blood brain barrier cross over
Effects bacterial replication Sulfonamides
Sulfonamides such as RIFAMPIN is used specifically for Mycobacterium
Interfere with DNA synthesis by making DNA ineffective Nalidixic acid and Quinolones
Disk Diffusion Method Kirby Bauer
Bacterial suspension containing 1.5 x 10*8 CFU/ml McFarland Standard
Cation concentration on agar is important due to Mg and Ca Mueller-Hinton agar
Lowest concentration of antimicrobial agent required to inhibit visible growth Minimal Inhibitory Concentration (MIC)
Lowest concentration of antibiotic that kills 99.9% growth Minimal Bactericidal concentration (MBC)
Must be drawn 15 min before dose Trough
Must be drawn 30-60 min after dose given Peak
Strip impregnated with antibiotics with scale for measurment E test
Created by: geebs28