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Histopathology Lab
Exam
| Question | Answer |
|---|---|
| Ehrlich Staining Time | 15-40 Minutes |
| Ehrlich Color: | Purple |
| Hematoxylin Staining Time | 5-10 Minutes |
| Hematoxylin color | Purplish Blue |
| Ripening of Mayer: | Sodium Iodate |
| The block is allowed to harden for cutting proper by facing them down in ice cold water or refridgerator for : | 5-10 Minutes |
| Sections are cut between - for routine histologic procedures | 4-6 u (micrometers) |
| The sections are floated out on a Water Bath set at | 45-50 C |
| Sections may be flattened out by placing them on a slide flooded with _ producing convection currents that will serve to _ | 20% alcohol, to remove the creases. |
| Floatation should expand the section to its original dimensions and ensure that it is completely flat. the temperature will need to be at _ | 5-9 C below the melting point of wax |
| After draining, the sections are fixed to the slides. This can be done either by leaving the slides in a | 37 C Incubator Overnight |
| Placing the slides at a wax oven at _ or drying the slides on a hot plate at | 56-60 C for 2 hours,45-55 for 30 to 45 Minutes |
| 1. Mayers' Egg Albumin Formula: | Egg White 50 CC Glycerin 50 CC Filter and add 100 mg of crystals of thymol to prevent the growth of molds. |
| Glycerin Jelly | (KAISER'S 1880) |
| REFRACTIVE INDEX (Glycerin Jelly) | 1.47 |
| FARRANT'S MEDIUM (Refractive Index) | 1.43 |
| FORMULA: (Farrant's Medium) | GUM ARABIC 50 GM. Distilled water 50 ml. Glycerol 50 ml. |
| APATHY'S MEDIUM (Refractive Index) | 1.52 |
| The pH of the medium is near | 4.0 (HIGHLY ACIDIC) so stains fade or bleed into the medium. |
| BRUN'S FLUID (Formula) | GLUCOSE 24 GM. GLYCERINE 6 ML. SPIRITS OF CAMPHOR 6 ML. DISTILLED WATER 84 ML. |
| is recommended for mounting frozen sections from water. | BRUN'S FLUID |
| CANADA BALSAM (Refractive Index) | 1.524 |
| is a natural resin extracted from the Canadian tree, | Canada Balsam |
| Usually dissolved in xylene in an incubator at 37°C or paraffin oven at 58 °C, and filtered, obtaining the desired consistency by controlled evaporation of the solvent. | ABUS BALSAMEA, |
| DPX (Refractive Index): | 1.532 |
| XAM (Refractive Index): | 1.52 |
| Is a synthetic resin mixture in xylene, available in a PALE YELLOW OR COLORLESS SOLUTION. | Xam |
| or phycobiliprotein fluorescent labels (phycoerythrin, phycocyanin) must not contain glycerol as this quenches the staining intensity. | Aqueous Mounting Media |
| Is the most aqueous mountant for fluorescent microscopy, being virtually non-fluorescent. | APATHY’S MEDIUM |
| The coverslip can be removed by: _ and placing the broken slide in the incubator at 37C until the mountant has been removed. | Soaking in Xylene |
Created by:
Jahad000
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