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Calculations

ASCP calculations

QuestionAnswer
TSAT (Transferrin saturation) [TSAT= (Fe/TIBC) x 100] or [TSAT=(Fe/TF) X 70.9]
TIBC UIBC + Serum Iron = TIBC
UIBC TIBC - Iron = UIBC
Indirect bilirubin (unconjugated) Total bilirubin - Direct bilirubin
LDL ( Friedewald) Cholesterol - HDL - (TG/5) = LDL
Non-HDL Cholesterol-HDL = Non HDL
A/G Ratio Albumin / (total protein-albumin)
Anion Gap Na - (Co2 + Cl)
Creatine clearance calculation (urine creatinine/serum creatinine) x ( TV(mls)/collection time) x (1.73/BSA)
Beers Law A = εlc A = absorbance , ε= constant, molar absorptivity, l= path length c= concentration
Corrected WBC counts when > 10 nRBCs present observed WBC count x (100 ÷ [nRBC + 100])
Manual hemocytometer counts 1. Take average cell count from the four sets of 16 squares 2. Multiply that number by 10,000 3. Multiply by 5 ( to correct from dilution)
Mean cell volume (MCV) [hematocrit % x 10] / RBC
Mean cell hemoglobin (MCH) [Hb x 10] / RBC
Mean cell hemoglobin concentration ( MCHC) [Hb x 100] / [Hematocrit %]
BSA Square root ((Ht (cm) x Wt (kg))/3600)
Estimated average glucose (28.7 x A1C) -46.7
Globulin Total Protein - albumin
Relative index (if total CK > 100) [(CK-MB)/CK] x100
VLDL Chol - HDL - LDL
24 Hour Urine Amylase Urine amylase x (total volume/1000)
24 Hour Urine Chloride Urine chloride x (total volume/1000)
24 Urine Creatinine [Urine Creatinine x (total volume/100)] / wght (kg)
Urine Malb/Creatnine ratio (Urine malb/urine Creatinine) x 100
24 Hour Osmol Urine Osmo x (Total volume/1000)
Created by: ariannalynne98
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