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Laboratory Managemen

Specimen collection and processing of microbiology

QuestionAnswer
For delays in transpot, most microbiologic specimens should be refrigerated except Blood / CSF / Anaerobic specimens
Specimens not routinely accepted for anaerobic culture are Sputum / Catheterized urine / Bowel contents
The temperature of incubation usually is 35C for bacteria and viruses and 30C for fungi. TRUE
No more than four blood culture sets should be submitted in a 24-hour period. TRUE
The optimal time to collect a blood sample for Loaloa extremities is Noon
With a duration of symptoms of <25-hours, thr probable infection causing meningities is Pyogenic bacteria
Anaerobic culture is not routinely performed on cerebrospinal fluid specimens. TRUE
The VDRL (Venereal Disease Research Laboratory tests)is the only useful test for detecting syphilis antibodies in CSF. TRUE
Otitis media most pften is caused by organisms derived from the respiratory flora. TRUE
Feces optimally should be examined within 2-hours of collection. TRUE
The reference method for detection of cytotoxine, such as the one produced by Clostridium difficile, is cell culture assay. TRUE
Fungal culture of stool is not recommended. TRUE
Organisms such as are always pathogenic in genital tract specimens. Chlamydia trachomatis and Haemophilus ducreyi but not Group B streptococci.
Culture of Actinomyces sp. should be incubated anaerobically for 14 days. TRUE
Trichomonas vaginalis most often is detected by examination of a wet mount examination of vaginal or urethral discharge, prostatic secretions, or urine sediment. TRUE
>10 squamous epithelial cells in a respiratory specimen is contaminated with saliva. TRUE
Legionellosis can be diagnosed by Culture / Detection of antigen in the urine / Serologic testing.
Burkholderia cepacia is an important respiratory pathogen in persons with cystic fibrosis. TRUE
No culture methods are available for Pneumocystis carinii. TRUE
Vincent angina is an acute necrotizing ulcerative tonsillitis. TRUE
Acceptable methods of urine collection include Midstream clean catch and Catheterization but not Foley catheter tip.
Most commonly isolated viruses from urine are Cytomegalovirus CMV, Herpes simplex virus HSV and Adenovirus.
Created by: 726273993