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#3 Questions
PASS ASCP QUESTIONS
| Question | Answer |
|---|---|
| Rh negative phenotype is most likely a result of | Rh D deletion |
| Serum ferritin levels in pregnant women is? | Lower than normal values |
| How does oral contraceptive change iron store levels? | Iron stores is higher -Ferritin is higher in oral contraceptives |
| On a automated coagulation analyzer,PT controls are in range but PTT controls are out of range. New controls produce same results. What do you do? | CaCl2 might have been added to APTT reagent accidentally, change reagents and repeat -Recombiplastin =PT reagent -CaCl2=APTT reagent |
| What enzyme is increased in Mumps? | Amylase -Salivary Amylase levels are elevated in Mumps |
| If the DAT is positive on the cord blood, what do you do next? | Antibody screen on maternal blood, if ABO type is unknown |
| What can cause false positive for uroblinogen on urine strips? | Porphobilinogen -any chemical that can react with Ehlrich's reagent can cause a false positive |
| Which carbohydrate is often used in the media plates to differentiate Staph Aureus from other Staphlococcus species, such as S. epidremidis? | Mannitol -Mannitol salt agar is a selective and differential medium for identification of S.aureus, produces yellow colonies by fermenting mannitol |
| What is the formula for Sensitivity? | TP\(TP+FN)X100 |
| In what blood group type expression of antigens is weakened or in some cases negative during pregnancy? | Lewis |
| What is the drug of choice for MRSA isolates? | Vancomycin |
| HPV test is more often done for what condition? | Genital Warts |
| Why early morning sputum specimens are recommended for TB testing? | More concentrated specimen, has higher yield |
| How do you differentiate between Primary and Secondary hypothyroidism? | TSH(Thyroid Stimulating Hormone) -Primary Hypothyroidism= increase in TSH decrease in T4 -Secondary Hypothyroidism= T4 decrease and TSH decrease or normal |
| How do RBC look like in concentrated urine samples? | They are crenated |
| How do you calculate coefficient of variation? | CV=SD/MEAN X 100 MEAN= average Mode=most frequent # Median=middle |
| In hemolytic anemia, how does bilirubin levels change? | Unconjugated bilirubin increase Urine urobilinogen increase |
| In blood bank DAT testing, 3 test tubes are negative to AHG when check cells is added, the result is still negative. What is the error? | Serum was omitted from the reaction |
| VMA test is done for diagnosis of what disease? | Neuroblastoma and Pheochromocytoma |
| What are Pappenheimer bodies? | Iron particles |
| Stray light can be detected in a spectrophotometer by utilizing a: | Sharp cut off filter |
| If the life span of RBC'S are shortened, how does the urine bilirubin levels change? | Urine bilirubin is neg Urine urobilinogen is increased |
| Hair perforation test is for identification of which 2 fungi? | Trichophyton Mentagrophyte and Tirubrum |
| In photometric method of measuring bilirubin, analyzers measure the color change at what wavelength? | 454nm |
| What enzyme can help with diagnosis of muscular dystrophy? | CPK(Creatine Phosphokinase) |
| AST/ALT ratio in a patient is significantly high. What does this suggest? | Alcoholic Liver Disease |
| 10 nucleated RBC 15,000 per mm3 WBC count What is the corrected WBC count? | 15000x100/10+100 uncorrected WBC X 100/nRBC+100 |
| Which of the following serves as the specimen of choice for the demonstration of tertiary Syphilis? | CSF -Test CSF-VDRL |
| What is the significance of eosinophils in urine? | Acute intestinal nephritis ->5% eos in urine is an indicator |
| A man showed up in a clinic w/ a CAT SCRATCH infection. The culture revealed gram negative pleomorphic rods. Colonies were very slow to grow. It is most likely? | Bartonella |
| Which of the following differentiate S.maltophilia from other non fermenter gram negative rods? | Oxidase Negative;DNase Positive -S.maltophilia=catalase positive and motile |
| A patients plasma was screened for alloantibodies and was positive for Anti K. What % of ABO compatible blood units would be K antigen negative and can be chosen for transfusion? | -91% -k 99.9% positive |
| What is Fletcher's media used for? | Leptospira |
| What test is used to confirm presence of Bence-Jones proteins? | Immunofixation |
| What is flocculation? | Is bridging and binding of destabilized solids into larger particles |
| When Primidone is administered, what other compound must be quantified in serum to monitor drug levels? | Phenobarbital |
| Which donor candidate wont be accepted for blood donation? | A 35 year old man who received hepatitis B immune globulin 6 months ago -Hb Ig Deferred 12 months -Tattoo 12 months -Autologous donors Hgb 11g/dL |
| Total Cholesterol is 220mg/dL HDL is 45mg/dL and Triglycerides is 180. What is the LDL level? | LDL=Total Cholesteral-HDL Cholesteral-Triglyceride/5 =151 |
| TIBC IS 200mcg/dL iron is 125mcg/dL. What is UIBC? | =75 -UIBC=TIBC-iron |
| Why would the color changes from blue to orange and then back to blue in clinitest? | Pass through Phenomenon |
| This catalase positive, gram positive bacilli w/diphteroid morphology is highly resistant to many antibiotics and is associated w/immunocompromised patients | Diptheriae -Corynebacterium, pertussis,throat |
| The best diagnostic test for excessive alcoholic consumption : | GGT= ALCOHOLIC LIVER DAMAGE |
| The growth of which of the following fungi will be enhanced by olive oil? | Malassezia furfur |
| Which mycobacterium is found in tap water? | M.gordonae -in soil and water |
| What blood group reagent deteriorate faster while in use? | MNS |
| Detection of antigen in urine specimen can be used for which of the following pneumonia infections? | Legionella -Streptococcus pneumoniae -Histoplasma capsulatum |
| Hemolysis pattern of Streptococcus pneumoniae: | Alpha Hemolytic -Gram positive, dark and green colony |
| What are Howell -Jolly bodies? | DNA Fragments -gram positive , dark and green colony |