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Success: Chemistry

Clinical chemistry questions from Success! in Clinical Chemistry

QuestionAnswer
"Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR, and near UV regions of the spectrum?<br>a. Tungsent-filament<br>b. Hydrogen<br>c. Deuterium<br>d. Mercury vapor<br>" Tungsent-filament This is a test
Whis of the following isolates light within a narrow region of the spectrum?<br>a. Photomultiplier tube<br>b. Monochromator<br>c. Photovoltaic cell<br>d. Detector<br> Monochromator
Which of the following is <i>not</i> descriptive of a photomultiplier tube?<br>a. Emits electrons proportionally to initial light absorbed<br>b. Must be shielded from stray light<br>c. Cannot be used with a chopper<br>d. Amplifies the initial signal rec Cannot be used with a chopper
Which of the following is <i>false</i> about a photomultiplier<br>a. Converts radiant energy (light) to electrical energy (current)<br>b. Amplifies the current significantly<br>c. Has a very rapid response time<br>d. Is composed of an iron plate and a l Is composed of an iron plate and a layer of selenium
Which type of photodector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis?<br>a. Photomultiplier tube<br>b. Phototube<br>c. Barrier layer cell<br>d. Photodiode array<br> Photodiode array
"When performing spectrophotometer quality assurance checks, what is the holmium oxide glass filter used to access?<br>a. Linearity<br>b. Stray light<br>c. Absorbance accuracy<br>d. Wavelength accuracy<br>" Wavelength accuracy
"In spectrophotometric analysis, what is the purpose of the reagent blank?<br>a. Correct for interfering chromogns<br>b. Correct for lipemia<br>c. Correct for protein<br>d. Correct for color contribution of the reagents<br>" Correct for color contribution of the reagents
"In regards to bichromatic analysis, which of the following is <i>false</i>?<br>a. Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength<br>b. Eliminates background interferences<br>c. Sample concen Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength
"The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the wavelengths that are permitted to impinge on the sample will be within what wavelength range?<br>a. 530-540 nm<br>b. 530-550 nm<br>c. 535-545 nm<br>d. 540-550 nm<br>" 535-545 nm
Which of the following formulas is an expression of the Beer-Lambert law that is routinely applied to spectrophotometric analysis?<br>a. A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub><br>b. C<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = A<s A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub>
"In spectrophotometry, which of the following is a mathematical expression of the relationship between absorbance and transmittance?<br>a. A = <i>abc</i><br>b. A<sub>u</sub>/C<sub>u</sub> = A<sub>s</sub>/C<sub>s</sub><br>c. A = 2 - log %T<br>d. A = log A = 2 - log %T
Which of the followining is <i>not</i> a problem inherent in turbidimetry?<br>a. Variation in particle size of samples<br>b. Variation in particle size of standards<br>c. Rate of aggregation or settling of particles<br>d. Need to maintain a constant and Need to maintain a constant and specific temperature
"Which of the following may be associated with reflectance spectrophotometry as it relates to the dry reagent slide technique?<br>a. Light projected to the slide at 180°-angle<br>b. Dye concentration directly proportional to reflectance<br>c. Unabsorbed "Unabsorbed, reflected light detected by photodetector"
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of the emitted light. What is the purporse of this design?<br>a. Prevent loss of emitted light<br>b. Prevent loss of the excitation light<br>c. Focus emitte Prevent excitation light from reaching the detector
Which of the following represents a primary advantage of performing fluorometric over absorption spectroscopic methods of analysis?<br>a. Increased specificity and increased sensitivity<br>b. Increased specificity and decreased sensitivity<br>c. Purity Increased specificity and increased sensitivity
Which of the following may be associated with fluorescence polarization?<br>a. Plane-polarized light is used for sample excitation<br>b. Small molecular complexes show a greater amount of polarization<br>c. It's a heterogenous technique employed in fluo Plane-polarized light is used for sample excitation
Which of the following may be associated with bioluminescence?<br>a. Light emission produced due to enzymatic oxidation of a substrate<br>b. Less sensitive than direct fluorescent assays<br>c. Electron excitation caused by radiant energy<br>d. Employs a Light emission produced due to enzymatic oxidation of a substrate
Nephelometry is based on the measurement of light that is:<br>a. Absorbed by particles in suspension<br>b. Scattered by particles in suspension<br>c. Produced by fluorescence<br>d. Produced by excitation of ground-state atoms<br> Scattered by particles in suspension
Which of the following instruments is used in clinical or reference laboratories to detect beta and gamma emissions?<br>a. Fluorometer<br>b. Nephelometer<br>c. Scintillation counter<br>d. Spectrophotometer<br> Scintillation counter
Which of the following best describes chemiluminescence?<br>a. Electron excitation caused by radiant energy<br>b. Enzymatic oxidation of a substrate produces light emission<br>c. Chemical energy excites electrons that emit light upon return to ground st Chemical energy excites electrons that emit light upon return to ground state
Which of the following isolates light within a narrow region of the spectrum?<br>a. Photomultiplier tube<br>b. Monochromator<br>c. Photovoltaic cell<br>d. Detector<br> Monochromator
Which of the following is <i>not</i> descriptive of a photomultiplier tube?<br>a. Emits electrons proportionally to initial light absorbed<br>b. Must be shielded from stray light<br>c. Cannot be used with a chopper<br>d. Amplifies the initial signal recei Cannot be used with a chopper
Which of the following is <i>false</i> about a photomultiplier<br>a. Converts radiant energy (light) to electrical energy (current)<br>b. Amplifies the current significantly<br>c. Has a very rapid response time<br>d. Is composed of an iron plate and a lay Is composed of an iron plate and a layer of selenium
Which type of photodector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis?<br>a. Photomultiplier tube<br>b. Phototube<br>c. Barrier layer cell<br>d. Photodiode array<br> Photodiode array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass filter used to access?<br>a. Linearity<br>b. Stray light<br>c. Absorbance accuracy<br>d. Wavelength accuracy<br> Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank?<br>a. Correct for interfering chromogns<br>b. Correct for lipemia<br>c. Correct for protein<br>d. Correct for color contribution of the reagents<br> Correct for color contribution of the reagents
In regards to bichromatic analysis, which of the following is <i>false</i>?<br>a. Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength<br>b. Eliminates background interferences<br>c. Sample concentra Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the wavelengths that are permitted to impinge on the sample will be within what wavelength range?<br>a. 530-540 nm<br>b. 530-550 nm<br>c. 535-545 nm<br>d. 540-550 nm<br> 535-545 nm
Which of the following formulas is an expression of the Beer-Lambert law that is routinely applied to spectrophotometric analysis?<br>a. A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub><br>b. C<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = A<sub A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub>
In spectrophotometry, which of the following is a mathematical expression of the relationship between absorbance and transmittance?<br>a. A = <i>abc</i><br>b. A<sub>u</sub>/C<sub>u</sub> = A<sub>s</sub>/C<sub>s</sub><br>c. A = 2 - log %T<br>d. A = log %T< A = 2 - log %T
Which of the followining is <i>not</i> a problem inherent in turbidimetry?<br>a. Variation in particle size of samples<br>b. Variation in particle size of standards<br>c. Rate of aggregation or settling of particles<br>d. Need to maintain a constant and s Need to maintain a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to the dry reagent slide technique?<br>a. Light projected to the slide at 180°-angle<br>b. Dye concentration directly proportional to reflectance<br>c. Unabsorbed, r Unabsorbed, reflected light detected by photodetector
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of the emitted light. What is the purporse of this design?<br>a. Prevent loss of emitted light<br>b. Prevent loss of the excitation light<br>c. Focus emitte Prevent excitation light from reaching the detector
Which of the following represents a primary advantage of performing fluorometric over absorption spectroscopic methods of analysis?<br>a. Increased specificity and increased sensitivity<br>b. Increased specificity and decreased sensitivity<br>c. Purity of Increased specificity and increased sensitivity
Which of the following may be associated with fluorescence polarization?<br>a. Plane-polarized light is used for sample excitation<br>b. Small molecular complexes show a greater amount of polarization<br>c. It's a heterogenous technique employed in fluo Plane-polarized light is used for sample excitation
Which of the following may be associated with bioluminescence?<br>a. Light emission produced due to enzymatic oxidation of a substrate<br>b. Less sensitive than direct fluorescent assays<br>c. Electron excitation caused by radiant energy<br>d. Employs a r Light emission produced due to enzymatic oxidation of a substrate
Nephelometry is based on the measurement of light that is:<br>a. Absorbed by particles in suspension<br>b. Scattered by particles in suspension<br>c. Produced by fluorescence<br>d. Produced by excitation of ground-state atoms<br> Scattered by particles in suspension
Which of the following instruments is used in clinical or reference laboratories to detect beta and gamma emissions?<br>a. Fluorometer<br>b. Nephelometer<br>c. Scintillation counter<br>d. Spectrophotometer<br> Scintillation counter
Which of the following best describes chemiluminescence?<br>a. Electron excitation caused by radiant energy<br>b. Enzymatic oxidation of a substrate produces light emission<br>c. Chemical energy excites electrons that emit light upon return to ground stat Chemical energy excites electrons that emit light upon return to ground state
Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR, and near UV regions of the spectrum?<br>a. Tungsent-filament<br>b. Hydrogen<br>c. Deuterium<br>d. Mercury vapor<br> Tungsent-filament This is a test too
Which of the following isolates light within a narrow region of the spectrum?<br>a. Photomultiplier tube<br>b. Monochromator<br>c. Photovoltaic cell<br>d. Detector<br> Monochromator
Which of the following is <i>not</i> descriptive of a photomultiplier tube?<br>a. Emits electrons proportionally to initial light absorbed<br>b. Must be shielded from stray light<br>c. Cannot be used with a chopper<br>d. Amplifies the initial signal recei Cannot be used with a chopper
Which of the following is <i>false</i> about a photomultiplier<br>a. Converts radiant energy (light) to electrical energy (current)<br>b. Amplifies the current significantly<br>c. Has a very rapid response time<br>d. Is composed of an iron plate and a lay Is composed of an iron plate and a layer of selenium
Which type of photodector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis?<br>a. Photomultiplier tube<br>b. Phototube<br>c. Barrier layer cell<br>d. Photodiode array<br> Photodiode array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass filter used to access?<br>a. Linearity<br>b. Stray light<br>c. Absorbance accuracy<br>d. Wavelength accuracy<br> Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank?<br>a. Correct for interfering chromogns<br>b. Correct for lipemia<br>c. Correct for protein<br>d. Correct for color contribution of the reagents<br> Correct for color contribution of the reagents
In regards to bichromatic analysis, which of the following is <i>false</i>?<br>a. Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength<br>b. Eliminates background interferences<br>c. Sample concentra Absorbance is measured at the spectral absorbance peak for a blank and the samples using the same wavelength
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the wavelengths that are permitted to impinge on the sample will be within what wavelength range?<br>a. 530-540 nm<br>b. 530-550 nm<br>c. 535-545 nm<br>d. 540-550 nm<br> 535-545 nm
Which of the following formulas is an expression of the Beer-Lambert law that is routinely applied to spectrophotometric analysis?<br>a. A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub><br>b. C<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = A<sub A<sub>u</sub> x C<sub>s</sub>/A<sub>s</sub> = C<sub>u</sub>
In spectrophotometry, which of the following is a mathematical expression of the relationship between absorbance and transmittance?<br>a. A = <i>abc</i><br>b. A<sub>u</sub>/C<sub>u</sub> = A<sub>s</sub>/C<sub>s</sub><br>c. A = 2 - log %T<br>d. A = log %T< A = 2 - log %T
Which of the followining is <i>not</i> a problem inherent in turbidimetry?<br>a. Variation in particle size of samples<br>b. Variation in particle size of standards<br>c. Rate of aggregation or settling of particles<br>d. Need to maintain a constant and s Need to maintain a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to the dry reagent slide technique?<br>a. Light projected to the slide at 180°-angle<br>b. Dye concentration directly proportional to reflectance<br>c. Unabsorbed, r Unabsorbed, reflected light detected by photodetector
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of the emitted light. What is the purporse of this design?<br>a. Prevent loss of emitted light<br>b. Prevent loss of the excitation light<br>c. Focus emitted Prevent excitation light from reaching the detector
Which of the following represents a primary advantage of performing fluorometric over absorption spectroscopic methods of analysis?<br>a. Increased specificity and increased sensitivity<br>b. Increased specificity and decreased sensitivity<br>c. Purity of Increased specificity and increased sensitivity
Which of the following may be associated with fluorescence polarization?<br>a. Plane-polarized light is used for sample excitation<br>b. Small molecular complexes show a greater amount of polarization<br>c. It's a heterogenous technique employed in fluoro Plane-polarized light is used for sample excitation
Which of the following may be associated with bioluminescence?<br>a. Light emission produced due to enzymatic oxidation of a substrate<br>b. Less sensitive than direct fluorescent assays<br>c. Electron excitation caused by radiant energy<br>d. Employs a r Light emission produced due to enzymatic oxidation of a substrate
Nephelometry is based on the measurement of light that is:<br>a. Absorbed by particles in suspension<br>b. Scattered by particles in suspension<br>c. Produced by fluorescence<br>d. Produced by excitation of ground-state atoms<br> Scattered by particles in suspension
Which of the following instruments is used in clinical or reference laboratories to detect beta and gamma emissions?<br>a. Fluorometer<br>b. Nephelometer<br>c. Scintillation counter<br>d. Spectrophotometer<br> Scintillation counter
Which of the following best describes chemiluminescence?<br>a. Electron excitation caused by radiant energy<br>b. Enzymatic oxidation of a substrate produces light emission<br>c. Chemical energy excites electrons that emit light upon return to ground stat Chemical energy excites electrons that emit light upon return to ground state
Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR, and near UV regions of the spectrum?<br>a. Tungsent-filament<br>b. Hydrogen<br>c. Deuterium<br>d. Mercury vapor<br> Tungsent-filament
Created by: ykosoko