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MLT-Dx of virus
Virology
Question | Answer |
---|---|
What is the storage condition for viral specimen? | 4 degree celsius and moist |
What is the transport medium for viral specimen? | Virus transport medium |
What are the 3 types of cell line to do the viral cell culture? | 1.Primary 2.Semi-continuous 3.Continous |
What are the example of primary cell lines? | 1.RMK (Rhesus monkey kidney) 2.RK (rabbit kidney) |
What is the advantage of primary cell line? | many RNA viruses & enteroviruses are easily isolated |
What are the main component of semi-continuous cell lines? | 1.Human embryonic fibroblast (HEF) 2.human diploid fibroblast (HDF) |
What the examples of semi-continuous cell lines? | 1.MRC-5 2.WI-38 |
What virus can be cultured in semi-continuous cell line? | 1.Rhinovirus 2.Cytomegalovirus |
What is the main component of continuous cell line? | malignant or mutated cells |
What are the examples of continuous cell line? | 1.HeLa (ca cervix), 2.HEp-2 (ca larynx) 3.Vero (African green monkey kidney) |
What is the incubation temperature for rhinoviruses, Influenza virus or Coronavirus? | 33 degree celsius |
What are the cytopathic effect that occur in monolayer indicate presence of viruses? | 1.Shrinking 2.Swelling 3.Syncytia (fused cells) 4.Inclusion bodies |
What are the two ways to observe the cytopathic effects? | 1.Microscope 2.Fix & Stain infected cell culture |
What are the 3 stains used to stain the infected cell? | 1.Giemsa 2.haematoxylin & eosin 3.fluorescent antibody |
What are the 2 categories of non-culture methods for viral identification? | 1.Viral antigen detection 2.Anti-virus antibody |
What are the 4 examples of viral antigen detection? | 1.Electron microscope 2.Enzyme Immuno Assay 3.ImmunoFluoresence 4.Complement fixation test |
Viral antigen detection is useful for non-culturable viruses, like _______ and ________? | 1.hepatitis B virus 2.rotavirus |
What are the 2 culture cell examples for influenza virus? | 1.LLC-MK 2.MDCK |
With observed cytopathic effects, which test to confirm the viral infection? | Immunofluorescence Test |
What are the 3 useful markers for Hepatitis B virus during window period? | 1.Anti-HBc IgG 2. IgM 3.Anti-HBe |
What are the 3 pros and 3 cons of Viral culture? | Pros: 1.Specific 2.Can tell viruses are active or not 3.can keep viral strain Cons: 1.Slow 2.some virus cannot be cultured 3. only viable virus |
What are the 3 pros and 3 cons of nucleic acid based assays? | Pros: 1.Specific 2.Sensitive 3.Rapid Cons: 1.risk of nucleic acid contamination 2.Cannot tell virus is active or not |
For viral family specific detection, which parts of the viral genome will be considered, why and example? | 5’ & 3’ untranslated regions, conserved within family, Flaviviridae |
For viral species specific detection, which parts of the viral genome will be considered, why and example? | 1. pol & gag genes, conserved within species, Dengue virus & Hepatitis C virus |
Which cancer is associated with Human papillomavirus, HPV (type 16)? | Cervical cancer |
Which cell is for the culture of HPV? | SiHa cells |
When to use SYBR Green? | 1.Screening of target DNA 2.When the PCR system is fully optimized |
When not to use SYBR Green? | 1.Multiplex reaction 2.Low conc of pathogens in the specimen 3.Amplification of rare transcript |
What can be used to increase the hybridization specificity? | Taqman probe |
What are the 3 components of a taqman probe? | 1.Reporter 2.Primer 3.Quencher |
When to use fluorescent probes? | 1.Low level pathogen detection, 2.Requires quantification |
What is the automation machine for HIV 1 detection? | NucliSens EasyQ system by bioMérieux |
What is the target of NucliSens EasyQ system for the detection of HIV 1? | gag sequence |
What is the detection limit of NucliSens EasyQ system? | as low as 25 copies/ml |