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MLT-Pasteurellaceae
Microbiology
| Question | Answer |
|---|---|
| Pasteurellaceae is facultative _______? | anaerobic |
| What is the gram stain observation of Pasteurellaceae? | Gram negative coccobacilli |
| Pasteurellaceae is motile ____A_____, catalase ______B______ and oxidase ____C_____. | 1. negative 2.positive 3.positive |
| Can Pasteurellaceae grow on MacConky Agar? | No |
| What is the most important Haemophilius spp.?? | Haemophilius influenzae |
| Haemophilius influenzae can be mostly isolated from ______A_____ and _____B_____? | A.Sputum B.CSF culture |
| Haemophilius influenzae can be divided into _____A______ and ______B______ strains? | 1.unencapsulated (non-typable) 2. encapsulated (typeable) |
| How many different serotype of H. influenzae? | 6, a to f |
| What is the obligate human pathogen? | Haemophilus influenzae type b (Hib) |
| What is the Type b polysaccharide capsule? | polyribosylribitoal phosphate |
| What can be caused by H. influenzae? | Meningitis |
| What is the gram statin morphology of H. ducreyi ? | 1.Gram negative rods 2."School of fish" |
| What are the grow condition of H. ducreyi? | 1.Grow at 33C 2.Slow grow rate, incubate for up to 9 days 3.Requires X factor (Hemin) only |
| H. influenzae & H. ducreyi grows on _________? | chocolate agar |
| What is the growth supplement of H. ducreyi? | Isovitalex |
| How to identify Haemophilius? | 1.Based on X factor and V factor requirement 2.Porphyrin test |
| H. influenzae requires _____A______ and ______B_______, and Porphyrin ____C____? | A.X factor B.V factor C.Negative |
| H. ducreyi requires _____A______ only, and Porphyrin ______B______? | A. X factor B. Negative |
| What is the disease caused by H. ducreyi? | Chancroid (STD) |
| What is the purpose of Satellitism test? | To identify H. influenzae on 5% blood agar. |
| What is the principle of Satellitism test? | 1. Blood agar only has X factor, but no V factor 2. Staphylococcus aureus produce NAD as a metabolic byproduct 3. H. influenzae grows very close to the colonies of S. aureus |
| Pasteurellaceae family has 2 big groups, what are they? | 1. Haemophilius 2. Pasteurella |
| What is the most important Pasteurella spp? | P. multocida |
Created by:
kencho
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