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MLT-Pasteurellaceae

Microbiology

QuestionAnswer
Pasteurellaceae is facultative _______? anaerobic
What is the gram stain observation of Pasteurellaceae? Gram negative coccobacilli
Pasteurellaceae is motile ____A_____, catalase ______B______ and oxidase ____C_____. 1. negative 2.positive 3.positive
Can Pasteurellaceae grow on MacConky Agar? No
What is the most important Haemophilius spp.?? Haemophilius influenzae
Haemophilius influenzae can be mostly isolated from ______A_____ and _____B_____? A.Sputum B.CSF culture
Haemophilius influenzae can be divided into _____A______ and ______B______ strains? 1.unencapsulated (non-typable) 2. encapsulated (typeable)
How many different serotype of H. influenzae? 6, a to f
What is the obligate human pathogen? Haemophilus influenzae type b (Hib)
What is the Type b polysaccharide capsule? polyribosylribitoal phosphate
What can be caused by H. influenzae? Meningitis
What is the gram statin morphology of H. ducreyi ? 1.Gram negative rods 2."School of fish"
What are the grow condition of H. ducreyi? 1.Grow at 33C 2.Slow grow rate, incubate for up to 9 days 3.Requires X factor (Hemin) only
H. influenzae & H. ducreyi grows on _________? chocolate agar
What is the growth supplement of H. ducreyi? Isovitalex
How to identify Haemophilius? 1.Based on X factor and V factor requirement 2.Porphyrin test
H. influenzae requires _____A______ and ______B_______, and Porphyrin ____C____? A.X factor B.V factor C.Negative
H. ducreyi requires _____A______ only, and Porphyrin ______B______? A. X factor B. Negative
What is the disease caused by H. ducreyi? Chancroid (STD)
What is the purpose of Satellitism test? To identify H. influenzae on 5% blood agar.
What is the principle of Satellitism test? 1. Blood agar only has X factor, but no V factor 2. Staphylococcus aureus produce NAD as a metabolic byproduct 3. H. influenzae grows very close to the colonies of S. aureus
Pasteurellaceae family has 2 big groups, what are they? 1. Haemophilius 2. Pasteurella
What is the most important Pasteurella spp? P. multocida
Created by: kencho