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MLT-Legionella

Microbiology

QuestionAnswer
Legionellaceae is gram _________. negative, rods
Legionellaceae is strict ________. aerobic
Legionellaceae is oxidase ____1_____ , catalase _____2_____, motile ____3_____. 1.positive 2.positive(weak) 3.positive
Legionella is Fastidious requires ____1_____ and ____2______ to thrive. 1.cysteine 2.iron
What is the most important spp in Legionellaceae? Legionella pneumophila
What is the transmission route of Legionella pneumophila? 1.Associated with water systems 2.survive in the free water, biofilm & amoeba
What are the specimen for Legionella pneumonphila? 1.Sputum, keep cool to prevent over-growth 2.Tissue from post-mortem
What is the culture media for Legionella pneumophila? Buffered charcoal yeast extract with L cysteine & ferric compound
What is the culture condition for Legionella pneumophila? Grow at 35 C in CO2 for at least 2 3 days
What can be observed from the Legionella pneumophila culture? Colonies are small (1-2 mm), white, glistening, circular, smooth & has “ground glass”
Legionella pneumophila can be gram stain? No, it will stain poorly.
Legionella pneumophila is ______ to biochemical tests? Inert
DFA (direct fluorescent antibody) is ____1____ and _____2_____ to ID L. pneumophila. 1.sensitive 2.specific
L. pneumophila antigen can be detected in ? urine of infected subjects
Urine antigen detection is more _______ than DFA. sensitive
Urine antigen detection can only detect serological group _____A____ of L. pneumophila. 1
Created by: kencho