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MLT-Legionella
Microbiology
Question | Answer |
---|---|
Legionellaceae is gram _________. | negative, rods |
Legionellaceae is strict ________. | aerobic |
Legionellaceae is oxidase ____1_____ , catalase _____2_____, motile ____3_____. | 1.positive 2.positive(weak) 3.positive |
Legionella is Fastidious requires ____1_____ and ____2______ to thrive. | 1.cysteine 2.iron |
What is the most important spp in Legionellaceae? | Legionella pneumophila |
What is the transmission route of Legionella pneumophila? | 1.Associated with water systems 2.survive in the free water, biofilm & amoeba |
What are the specimen for Legionella pneumonphila? | 1.Sputum, keep cool to prevent over-growth 2.Tissue from post-mortem |
What is the culture media for Legionella pneumophila? | Buffered charcoal yeast extract with L cysteine & ferric compound |
What is the culture condition for Legionella pneumophila? | Grow at 35 C in CO2 for at least 2 3 days |
What can be observed from the Legionella pneumophila culture? | Colonies are small (1-2 mm), white, glistening, circular, smooth & has “ground glass” |
Legionella pneumophila can be gram stain? | No, it will stain poorly. |
Legionella pneumophila is ______ to biochemical tests? | Inert |
DFA (direct fluorescent antibody) is ____1____ and _____2_____ to ID L. pneumophila. | 1.sensitive 2.specific |
L. pneumophila antigen can be detected in ? | urine of infected subjects |
Urine antigen detection is more _______ than DFA. | sensitive |
Urine antigen detection can only detect serological group _____A____ of L. pneumophila. | 1 |