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MLT-Mol Diagnostic
Microbiology
Question | Answer |
---|---|
What is the protein based method for bacterial identification in advanced diagnostic? | MALDI-TOF MS |
What are the Nucleic acid based method for bacterial identification in advanced diagnostic? | 1.Species-specific PCR (IS6110PCR for M. tuberculosis) 2.Broad range PCR |
What are the Biochemical based method for bacterial identification in advanced diagnostic? | 1.Vitek 2 2.Phoenix 100 |
What are the methods for drug resistance detection in advanced diagnostic? | 1.Resistance gene specific PCR 2.PCR sequencing 3.MALDI-TOF MS |
Prokaryotic ribosomes consist of ___A___ subunit (small sub unit) and ____B____subunit (large sub unit) -> complete ____C_____ ribosome | A:30S B:50S C:70S |
What is the composition of 30S subunit? | 1.16S ribosomal RNA 2. 21 proteins |
The gene coding for 16s ribosomal RNA is referred to as _______? | 16S rDNA |
What are the 2 characteristics making 16S rDNA ideal for bacterial identification? | 1.Highly conserved 5' and 3': Universal primers 2.The central region is hypervariable |
What are the limitation of 16s rRNA sequencing? | 1. Require 2-3 days for the entire workflow 2.Cannot differentiate similar species, like Mycobacterium spp. 3.Easy to be contamined by environmental bacterial 4.Post PCR leads to aerosol, and hence contamination |
What are the 3 spatial separation for PCR set up? | 1.Area1: Mastermix preparation 2.Area2:Specimen preparation / DNA extraction 3.Area3:Amplification and detection |
What is the commercial PCR based test for MTB? | Xpert MTB/RIF |
What is the target gene for Xpert MTB/RIF to amplify? | rpoB gene, specific to M. tuberculosis |
rpoB gene encodes ______? | Target for rifampicin |
Mutation of rpoB gene will lead to ? | 90% rifampicin resistance |
What is the function of Xpert MTB/RIF? | 1.Qualitative detection of M. tuberculosis 2.Detect the rpoB mutation |
What is FilmArrayBlood Culture Identification Panel? | 1. A nested multiplexes PCR 2. Can detect 24 pathogens + 3 antibiotic resistance genes 3. Bloodstream infection |
How many stage of PCR will FileArray PCR perform? | 2 |
What is the purpose of first stage PCR of FilmArray? | a single, large volume, massively multiplexed reaction |
What is the purpose of second stage PCR of FilmArray? | to detect the products from the first stage PCR |
How to detect the reaction of the FilmArray? | intercalating dye and end-point melting curve |