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MLT-CSF culture
Microbiology
Question | Answer |
---|---|
What are the causative pathogens of meningitis for neonate? | 1.GBS: Streptococcus agalactiae 2.E.coli 3.Listeria monocytogenes |
What are the causative pathogens of meningitis for children under 10? | 1.Haemophilius influenzae 2.Neisseria meningitis 3.Streptococcus pneumoniae |
What are the causative pathogens of meningitis for children > 10 or adults? | 1.Neisseria meningitis 2.Streptococcus pneumoniae |
What are the causative pathogens of meningitis for Immunosuppressed or severely leukopenic patient? | 1.Cryptococcus neoformans–fungus 2.Listeria monocytogenes 3.Pseudomonas aeruginosa |
What are the parasites can be associated with Meningitis? | 1.Entamoeba histolytica 2.Toxoplasma gondii |
After CSF collection, it will be separated into 3 tubes, which are for? | 1.Biochemistry 2.Haematology 3.Microbiology |
What is the transport condition for CSF? | Room temperature. Most pathogens sensitive to cold condition, e.g. Neisseria meningitidis |
What is the staining method for CSF bacteria? | Gram stain |
What is the staining method for CSF fungi? | India ink (Negative stain) |
What are the staining methods for Mycobacteria? | AuramineO and ZN stain |
What is the staining method for parasite? | Wet mount smear |
What is the centrifugation condition for CSF concentration? | 2000 rpm for 10mins |
What is the usage of sediment and the supernatant after centrifugation? | 1.sediment: culture and smear 2.supernatant: serological studies for antigens |
If indian ink is positive, which agar will be used to do subculture? | Sabouraud agar |
What is LJ media and its function? | 1.Löwenstein–Jensen medium 2.For culture of Mycobacterium species, Mycobacterium tuberculosis |
When to use LJ medium? | When AO/ ZN stain positive |
What is the rapid diagnosis of CSF? | Direct Antigen Detection Test using Latex Agglutination principal. |
What are the examples of direct antigen detection test? | 1.BD Directigen for Meningitis Test 2.CryptococcalAntigen Latex Agglutination System (CALAS) |
BD Directigen is direct qualitative detection of antigens of? | 1.H. influenzae type b 2.Streptococcus pneumoniae 3.GBS 4.N. meningitidisgroup A, B, C, Y and W135 5.E.coli K1 |