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MLT-CSF culture

Microbiology

QuestionAnswer
What are the causative pathogens of meningitis for neonate? 1.GBS: Streptococcus agalactiae 2.E.coli 3.Listeria monocytogenes
What are the causative pathogens of meningitis for children under 10? 1.Haemophilius influenzae 2.Neisseria meningitis 3.Streptococcus pneumoniae
What are the causative pathogens of meningitis for children > 10 or adults? 1.Neisseria meningitis 2.Streptococcus pneumoniae
What are the causative pathogens of meningitis for Immunosuppressed or severely leukopenic patient? 1.Cryptococcus neoformans–fungus 2.Listeria monocytogenes 3.Pseudomonas aeruginosa
What are the parasites can be associated with Meningitis? 1.Entamoeba histolytica 2.Toxoplasma gondii
After CSF collection, it will be separated into 3 tubes, which are for? 1.Biochemistry 2.Haematology 3.Microbiology
What is the transport condition for CSF? Room temperature. Most pathogens sensitive to cold condition, e.g. Neisseria meningitidis
What is the staining method for CSF bacteria? Gram stain
What is the staining method for CSF fungi? India ink (Negative stain)
What are the staining methods for Mycobacteria? AuramineO and ZN stain
What is the staining method for parasite? Wet mount smear
What is the centrifugation condition for CSF concentration? 2000 rpm for 10mins
What is the usage of sediment and the supernatant after centrifugation? 1.sediment: culture and smear 2.supernatant: serological studies for antigens
If indian ink is positive, which agar will be used to do subculture? Sabouraud agar
What is LJ media and its function? 1.Löwenstein–Jensen medium 2.For culture of Mycobacterium species, Mycobacterium tuberculosis
When to use LJ medium? When AO/ ZN stain positive
What is the rapid diagnosis of CSF? Direct Antigen Detection Test using Latex Agglutination principal.
What are the examples of direct antigen detection test? 1.BD Directigen for Meningitis Test 2.CryptococcalAntigen Latex Agglutination System (CALAS)
BD Directigen is direct qualitative detection of antigens of? 1.H. influenzae type b 2.Streptococcus pneumoniae 3.GBS 4.N. meningitidisgroup A, B, C, Y and W135 5.E.coli K1
Created by: kencho