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MLT-Blood Culture

Microbiology

QuestionAnswer
What is the bacteraemia? the presence of bacteria in the blood
What is Septicaemia(Sepsis)? Uncontrolled and continuous bacteraemia
Whats the effect of Septicaemia? 1.Induce systemic inflammatory response syndrome -> Organ failure 2. Vasodilation of major vessel -> Hypotension(Septic Shock) 3.Death
Why 1:10 dilution of patient’s blood in cultured medium is recommended? To minimize the inhibitory effect of normal serum or antibiotics on bacteria growth.
What are the two blood culture bottles? 1.BACTEC (BD Diagnostics) 2.BacT/Alert (bioMerieux)
Whats the content inside blood culture bottle? Soybean-casein digest broth supplemented with SPS and Resin
Whats the function of SPS in blood culture bottle? Sodium PolyanetholSulphonate 1. As anticoagulant 2. Inhibit complement and lysozyme activity 3. Interferes with phagocytosis 4. Inhibitory to Neisseria species
Whats the function of Resin in blood culture bottle? Adsorb antimicrobials, for patient who has started therapy
What are the type of blood culture bottle? 1. Aerobic bottle 2. Anaerobic bottle, with CO2, N2 and thiols 3. Pediatric bottle, with sodium pyruvate for fastidious organisms 4. Mycobacteria bottle, with agent to lyse RBC and WBC
How BACTEC and BacT/Alert systems to determine the bacterial growth? By monitoring the increase in CO2 content
Can the inoculated bottles be store in refrigeration? No
Whats the ways of BACTEC system to detect bacterial growth? 1. Radiometric method 2.Fluorescent method
How does BACTEC system Radiometric method work? detects bacterial growth by by measuring radiolabeled 14CO2 which is released from a 14Carbon radiolabeled substrate (e.g. glucose)
How does BACTEC system Fluorescent method work Sensors at bottom of bottle reacts with CO2, LED then activated, Photo-detector measures the level of fluorescence
Whats the mechanism of Bact/Alert to detect bacterial growth? Colorimetric method
How does the Bact/Alert colorimetric method work? Microorganisms multiply in the media, generating CO2. As CO2increases, the sensor in the bottle turns a lighter color
Whats the advantage of Bact/Alert colorimetric method? 1.Changes in the sensor are permanent 2.Visible to the unaided eye, unlike any other method
What are the incubation time (day) for the blood bottle culture system? 1. General patient: 5 2. Bacterial endocarditis patient: 14 3.Mycobacteriumculture in MycoLytic broth: 42
If there is alarm from the automatic blood culture system, what to do next? 1. Prepare direct blood smear in BSC 2. Gram staining and check the morphology
The Gram result can indicate for ? 1.Choice of antimicrobial agent for treatment 2.Selection of biochemical test for Bacterial ID
If aerobic blood bottle is positive, how to do subculture? 1.Blood agar: growth and check for hemolysis 2.Chocolate agar: with lysed RBC, for fastidious species, eg, Haemophilius, Neisseria 3.MacConkey agar: differentiate lactose and non lactose fermenter
If anaerobic blood bottle is positive, how to do subculture? 1.Blood agar 2.Chocolate agar 3.MacConkey agar 4.Anaerobic blood ager 5.Neomycin Blood agar
What is MH agar used in subculture? 1.Mueller-Hinton agar 2.commonly used for antibiotic susceptibility testing
Common organisms found in positive blood culture when the patients are not catheterized nor immunocompromised ? 1.Stap. epidermidis 2.Propionebacterium acnes 3.Bacillus spp 4.Corynebacteriumspp 5.Nonhaemolytic strept 6.Non-aeruginosalpseudomonas
What are the Significant Pathogens In General found in positive blood culture? 1.all Gram-negative:H. influenzae, N. gonorrheae. and E.coli 2.Stap. aureus 3.Strept. pneumoniae 4.GBS 5.Pseudomonas aeruginosa 6.Acinetobacterspp 7.Enterococcus spp 8.Listeria monocytogenes 9.Bacteriodes 10.Yeasts and fungi
Entire workflow of automative blood culture system to subculture need _____ hours to finish? 24-72
What are the example of Semi-automated biochemical bacterial ID system? 1.Vitek 2 2.Phoenix 100
How does Semi-automated biochemical bacterial ID system work? 1.prepare bacterial suspension with required turbidity 2.load the suspension to the plastic card that contains all biochem reactions to differentiate GP or GN 3.load the plastic card into the machine
What is the TAT of the Semi-automated biochemical bacterial ID system? 5-7 hours
Whats next after positive blood culture? 1. Do direct bacteria Identification using MALDI-TOF
Whats the total hands on time of MALDI-TOP per sample? 5 minutes
Created by: kencho