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Whats basic info of Vibrionaceae? 1.Gram negative rod 2.facultative aerobic 3.good growth of Mac 4.Oxidase positive 5.Halophilic
What is Mac agar? MacConkey Agar, a selective and differential agar
Why Mac is selective? Crystal violet and bile salts:no growth of Gram + bacteria and fastidious Gram - bacteria. Gram-negative enteric bacteria can tolerate bile salts because of their bile-resistant outer membrane.
Why Mac can use to differentiate? lactose fermenting gram-negative rods -> Purple, eg. Vibrionaceae non-lactose fermenting gram-negative rods -> Colourless
What are the species of Vibrio ? Vibrio cholerae, Vibrio parahaemolyticus, Aeromonas, Plesiomonas
What is 0129(150ug)? Vibrio static agent 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) . Vibrio is sensitive it. Aeromonas is resistant to it.
What is the reservoir of Vibrio? Shellfish
What would be caused by V.cholerae? gastroenteritis
What toxic would be produced by V.cholerae? Cholera toxin, it is a neurotoxin
The function of Cholera toxin? 1.Block uptake of Na and Cl from lumen 2.Lead to great loss of fluid and electrolyte(15-20L/day)
What is the transport medium for Vibrio? Transport medium: Cary Blair semi solid agar
What is the enrichment medium for Vibrio? Alkaline peptone broth, pH 8.4
What is the selective and differential culture medium for Vibrio? Thiosulphate Citrate Bile salt Sucrose Agar, TCBS V.cholerae: positive, yellow V.parahemolyticus: negative, green
Created by: kencho