click below
click below
Normal Size Small Size show me how
MLT-Neisseria
Microbiology
Question | Answer |
---|---|
What basic info of Neisseria? | 1.Gram negative 2.Aerobic 3.Cocci 4.Oxidase positive 5.Catalase positive |
What are the examples of Neisseria? | 1.Neisseria gonorrhoeae 2.Neisseria meningitidis |
What are the causes of Neisseria gonorrhoeae? | 1.Human pathogen 2.Infect mucosal surface 3.Gonorrhoea(STD) |
Where to get the specimen for Neisseria gonorrhoeae? | 1.Genital swabs, rectal swabs, eye swabs, joint fluid |
What are the medium for the specimen for Neisseria gonorrhoeae? | Specimen should be put in transport medium (Stuart/ Amies transport medium) |
What is direct examination of Neisseria gonorrhoeae? | Intracellular Gram negative diplococci |
What is GC agar? | GC Agar is used with hemoglobin and enrichment for the isolation and cultivation of Neisseria gonorrhoeae and other fastidious organisms. |
What is Thayer Martin medium? | 1.GC agar based 2.haemoglobin powder 3. Isovitalex 4.vancomycin, colistin, nystain |
What is modified TM medium? | 1.2% agar, 0.25% glucose & trimethroprim 2.inhibits growth & prevent swarming of Proteus |
What is the incubation condition for TM medium? | 37 C, 5-10% CO 2 , 2-3 d, sometimes 1 wk |
Where to get specimen for Neisseria meningitis? | CSF, blood, nasopharyngeal swab |
What is the medium for Neisseria meningitis? | blood & chocolate agar, Incubation: 37 C, 5-10% CO2 |
Where to find Kingella? | 1.Bacterial flora in the upper resp tract 2.Opportunistic pathogen(URTI, septicaemia) |
Kingella's catalase test result is? | negative |
Where to find Actinobacter? | 1.Normal skin 2.Nosocomial infection 3. |
Whats the tests results for Actinobacter? | GNCB, non fermenter*, very good growth on MacConkey agar (non lactose fermenter) Oxidase ve O/F test: oxidative non motile |
Where to find Moraxella? | In nasopharynx Opportunistic pathogen |
What infection is caused by M. catarrhalis? | Respiratory tract infection, eye infection |
How to lsolate Moraxella? | Isolation on blood & chocolate agar DNase +ve ; nitrate +ve |