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MLT-Neisseria

Microbiology

QuestionAnswer
What basic info of Neisseria? 1.Gram negative 2.Aerobic 3.Cocci 4.Oxidase positive 5.Catalase positive
What are the examples of Neisseria? 1.Neisseria gonorrhoeae 2.Neisseria meningitidis
What are the causes of Neisseria gonorrhoeae? 1.Human pathogen 2.Infect mucosal surface 3.Gonorrhoea(STD)
Where to get the specimen for Neisseria gonorrhoeae? 1.Genital swabs, rectal swabs, eye swabs, joint fluid
What are the medium for the specimen for Neisseria gonorrhoeae? Specimen should be put in transport medium (Stuart/ Amies transport medium)
What is direct examination of Neisseria gonorrhoeae? Intracellular Gram negative diplococci
What is GC agar? GC Agar is used with hemoglobin and enrichment for the isolation and cultivation of Neisseria gonorrhoeae and other fastidious organisms.
What is Thayer Martin medium? 1.GC agar based 2.haemoglobin powder 3. Isovitalex 4.vancomycin, colistin, nystain
What is modified TM medium? 1.2% agar, 0.25% glucose & trimethroprim 2.inhibits growth & prevent swarming of Proteus
What is the incubation condition for TM medium? 37 C, 5-10% CO 2 , 2-3 d, sometimes 1 wk
Where to get specimen for Neisseria meningitis? CSF, blood, nasopharyngeal swab
What is the medium for Neisseria meningitis? blood & chocolate agar, Incubation: 37 C, 5-10% CO2
Where to find Kingella? 1.Bacterial flora in the upper resp tract 2.Opportunistic pathogen(URTI, septicaemia)
Kingella's catalase test result is? negative
Where to find Actinobacter? 1.Normal skin 2.Nosocomial infection 3.
Whats the tests results for Actinobacter? GNCB, non fermenter*, very good growth on MacConkey agar (non lactose fermenter) Oxidase ve O/F test: oxidative non motile
Where to find Moraxella? In nasopharynx Opportunistic pathogen
What infection is caused by M. catarrhalis? Respiratory tract infection, eye infection
How to lsolate Moraxella? Isolation on blood & chocolate agar DNase +ve ; nitrate +ve
Created by: kencho