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What are the morphology and characteristics of Staphylococci? 1.Gram positive 2.Facultatively anerobic 3.Non motile and non-spore forming 4.catalase positive 5.Can growth in presence of 10% NaCl
Staphylococcus is divided into 2 groups by ? 1. Coagulase 2. S.aureus, coagulase positive 3. coagulase negative Staphylococci
What are the virulence factors for S.aureus? 1.Coagulase: clot blood 2.Exotoxin 3.Exfoliative toxin: scalded skin 4.Enterotoxin:induce intestinal fluid secretions 5.Enterotoxin type B: A superantigen, causes Toxic Shock Syndrome
What are the examples of coagulase negative Staphylococci? 1.S.epidemidis, normal flora on skin 2.S.saprophyticus, normal flora female genital tract 3.S. lugdunensis, skin flora
What are the tests for S.aures? 1. Yellow colonies on Mannitol salt agar, it ferments mannitol. Coagulase negative Stap is Red. 2.Solid coagulase test: positive 3.Tube coagulase test(Definitive result): positive 4.Staphaurex
How to do a tube coagulase test? 1.Suspend organism into ½ diluted rabbit plasma 2.Incubate the tube at 37℃ 3.Observe the results at 4 hours and 24 hours
What is Staphaurex? Latex agglutination test, it detects both clumping factor and Protein A on the surface of S.aureus
Is S.aures penicillin resistant? Yes, as it produces penicillinase.
What is the treatment for penicillin resistant S.aures? Methicillin-group antibiotics
What is Coagulase negative Stapylococci (CNS)? 1.Normal flora at different body parts 2.Opportunistic pathogens
What are the CNS? 1.Staphylococcus epidermidis(blood culture) 2.Staphylococcus saprophyticus(UTI) 3.Staphylococcus lugdunensis(blood culture) 4.Staphylococcus intermedicus(dog-bite)
What is Staphylococcus epidermidis? 1.Normal skin flora 2.the mostly frequently isolated CNS from bloodstream, insufficient skin disinfection prior to blood-taking 3. Up to 95% are methicillin resistant
What is Staphylococcus saprophyticus 1.Normal flora in female genital tract 2.10^5CFU/ml -> UTI
Novobiocin test is to differentiate? 1.Staphylococcus saprophyticus: Resistant 2.Staphylococcus epidermidis: Sensitive
Created by: kencho