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MLT-Primary Tests

Microbiology

QuestionAnswer
What is primary test? Test/observation performed directly on bacterial colonies. Rapid demonstration of the enzymes or motility.
What are the colony morphology description? circular, spindle, irregular, swarming(Proteus), mucoid(Klebsiella)
Oxygen condition for the growth of different type of microbes? 1.Strict aerobe - only grow with O2. 2.Facultative anaerobe - can grow with or without O2. 3.Microaerophilic - only grow with 5-7% O2. 4.Strict anaerobe - only grow without O2.
_______ found in Gram positive rod only? Spores
Spore forming bacteria are ______? Heat resistant
How to stain spore forming bacteria? Moeller's method, spores will be stained red by Carbol Fuchsin.
Another way to stain spore forming bacteria? Malachite Green method, spores will be stained green.
What is the indirect method to test the presence of spores? To test the survival of a culture after heating at 80 C for 10 min.
How to reveal the motility? Hanging drop method or semi-solid agar.
How catalase test works? A haemoprotein present in RBC that catalyses the breakdown of H2O2 to O2 and H2.
Catalase is used to differentiate ? Staphylococci : + ve Streptococci: - ve
How Cytochrome oxidase test works? Haemoproteins: transfer electrons to O2 in aerobic respiration. 1% tetramethyl p phenylenediamine di hydrochloride will be oxidized. Colourless to Deep purple.
Cytochrome oxidase test is used to test? Pseudomonas aeruginosa: +ve Escherichia coli:-ve
Created by: kencho