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MLT-Microbial Struct

Microbiology

QuestionAnswer
Microbial morphology and arrangement, what kind of cocci? 1.In pairs/chain 2.In packets: 3D packing perpendicular to one another 3.In cluster: 3D and random
Microbial morphology and arrangement, what kind of Bacilli/Rods? -Usually single rods. -Sometime short rods: coccobacilli
Microbial morphology and arrangement, what kind of vibrio? curved-rod shape (comma shape).
Microbial morphology and arrangement, what kind of spirochete/helical rods? spiral-shaped bacteria
The steps of grain stain? 1. Cells are stained with crystal violet dye 2.Iodine is added to form a complex between the crystal violet and iodine 3.Alcohol dehydrate the peptidoglycan layer, shrinking and tightening it 4.Safranin, a counter stain for Grain Neg.
Compare and contrast Grain stain Pos and Grain stain Neg. Grain Pos has 1 thick peptidoglycan layer, cell wall is thicker, with teichoic acid, purple in colour
Created by: kencho