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Biochemical tests
Question | Answer |
---|---|
Interpret a TSI/KIA tube with red slant and black butt after proper incubation? | K/A with H2S+, organism is glucose fermenter with hydrogen sulfide production |
What does R/A mean in a LIA tube? | Red slant over yellow butt, positive deamination of lysine (negative for decarboxylation) |
Decarboxylation of amino acids (lysine, ornithine) is an alkaline reaction which neutralizes the acidic reaction of utilizing glucose and reverts the media color back to its original color. T/F | True |
What should be present in the indole test tube in order to have a positive result? | Tryptophan |
Interpret the positive result of PA tube and what reagent is needed? | Dark green on slant's surface meaning organism can deaminate phenylalanine to phenylpyruvic acid. 10% ferric chloride |
Interpret a green acetamide tube? the indicator? | Organism is not able to utilize acetamide as a carbon source. Brom-thymol blue |
What makes hot pink color in urea tube? | Ammonia |
Interpret a red nitrate tube after adding sulfanilic acid, alpha-naphthylamine and zinc dust? | Negative for nitrate reduction |
Name two genera with cytochrome oxydase activity? | Pseudomonas and Neisseria |
Name the most probable genera on a positive cetrimide tube? | Pseudomonas |