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Enterobacteriaceae 2

Types of Testing: Amino Acid Utilization

Test NamePrinciple of TestProcess
Dehydrolization remove hydrogen Arginine - two step process = convert Ornithine then decarboxylated to give reaction
Deamination remove the amine (NH2) group Phenylalanine (occurs in the presence of oxygen) = reaction is demonstrated by addition of 10% ferric chloride to produce a green color
Lysine Iron Agar - LIA contains glucose, peptones, 2 pH indicators / provide a favorable environment for decarboxylation to take place = acid & anaerobic oil added after inocculation / purple color = positive reaction / black color = H2S production / All purple (K/K) = positive / Top purple/butt yellow (K/A) = negative / K/A (Top purple/butt black) = H2S positive / N/A (Top pink/butt yellow) = negative
Decarboxylase converts amino acids to amines - cleaves the carboxyl group from amino acid Lysine (amino acid) - Lysine decarboxylase - Cadaverine (amine) + CO2 Ornithine (amino acid) - Ornithine decarboxlyse - Putrescine (amine) + CO2
Created by: nbechen3