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GRAM POS. BACILLI
The Lord will bless me! Aja!
| Question | Answer |
|---|---|
| non spore forming gram pos bacilli | Corynebacterium and Listeria |
| contains endotoxin | Listeria |
| catalase (+) central/sub terminal not swollen | Bacillus ( aerobic at 25-30C) |
| catalase (-) terminal swollen spores | Clostridium (aerotolerant) |
| largest pathogenic bacteria | Bacillus anthracis |
| bamboo fishing rod | B. anthracis |
| boxcar (cylindrical central, sub terminal spores) | B. anthracis |
| non hemolytic with medusa head or beaten egg white colonies which is encapsulated and penicillin sensitive | B. anthracis |
| maybe medusa head but has a wide zone of beta hemolysis; Penicillin resistant | B. cereus |
| String of pearl test (35C) | B. anthracis |
| for infected animals ( gram pos.) | Ascoli Test |
| inverted pine growth ; slow liquefaction on gelatin | B. anthracis |
| implicated in food poisoning ; ground glass appearance | B. subtilis |
| for encapsulated strains | Fluorescent Antibody Staining |
| Woolsorter's disease | B. anthracis |
| VF: gamma D glutamic acid ; from herbivores (sheep, goats and cattles) | B. anthracis |
| fried rice endospores ; no treatment for food poisoning (resistant to beta lactam antibiotics) | B. cereus |
| compponent of the normal flora which is widespread in soil | Clostridium |
| tennis racket, or drumstick ; motile (also boxcar) | C. tetani |
| motile ; only lipase (+) clostridium | C. botulinum |
| double zone of hemolysis ; (+) Nagler's test using EYA | C. perfringens |
| phenylethyl alcohol with sheep's blood agar, BHI-S | Egg Yolk Agar (EYA) |
| excellent selective and differential media for C. dificile | Cycloserine Cefoxitin Fructose Agar (CCFA) |
| Lockjaw (Trismus) ; Risus sardonicus | C. tetani |
| inhibits release of GABA and glycine from nerve cells resulting to sustained muscle contraction | tetanospasmin |
| from stored vegetables, canned goods, ziplock storage bags, smoked fish and FRESH HONEY | C. botulinum |
| Infant botulism ; flaccid paralysis also food borne botulism | C. botulinum |
| Gas gangerene ; Pig bell and hyperbaric O2 treatment | c. perfringens |
| pseudomembranous colitis ; horse manure odor | C. dificile |
| Target hemolysis : complete theta toxin | inner zone |
| Target hemolysis: incomplete alpha toxin or lecithinase | outer zone |
| streaked perpendicular to a known group B streptococcus ; identifies C. perfringens | Reverse CAMP Test |
| (+) for reverse CAMP Test | arrowhead hemolysis |