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GRAM NEGATIVE COCCI
| Question | Answer |
|---|---|
| Gram neg cocci : aerobes | Neisseria and Branhamella/Moraxella |
| cytochrome oxidase is found on | CNAPP (Campylobacter, Neisseria, Aeromonas, Pseudomonas and Pasteurella) |
| pos for cytochrome oxidase | Neisseria |
| diplococci, kidney bean or coffee bean shape | Neisseria |
| two human pathogen of Neisseria | N. gonorrheae or N. meningitidis |
| part of normal upper respiratory tract flora | Moraxella catarrhalis |
| urogenital swabs: | plated bedside and transported to lab within 24hrs |
| for chlamydia : transport medium | 0.2 M sucrose phosphate ( 2SP ) |
| flat bottle coated with MTM under increased CO2 | Transgrow bottle |
| flat plastic dish with medium selective of gonococcus and tablet that acts as CO2 generator | JEMBEC AGAR |
| blood heated to 80°C to inactivate inhibitors present in BAP | CAP |
| enriched with iron, hemin (X factor) coenzyme 1 or NAD (V factor) | CAP |
| BA enriched with Hgb and supplements | Thayer's Martin (TM) |
| TM : inhibits gram positive organisms | Vancomycin |
| TM : inhibits gram negative organisms except Neisseria | Colistin |
| TM : inhibits fungi | Nystatin |
| same as TM plus trimethoprim | Modified Thayer Martin (MTM) |
| peptone agar base, cornstarch with yeast dialysate , 3% Hb horse plasma | New York City Agar (NYC) |
| with carbohydrates (glucose, maltose, sucrose, lactose and fructose) | Cystine Trypticase Acid Base (CTA MEDIUM) |
| CTA: positive control | N. gonorrheae |
| detect DNase activity in species of aerobic bacteria | Deoxyribonuclease (DNase Test) |
| indicato for DNAse Test | Methyl Green |
| (+) for Dnase test | S. marcescens and S. aureus (clear zone) |
| 30% H2O2 | Superoxol Test ( Catalase Test) |
| allows rapid detection of carbohydrate utilization and production of DNase and beta lactamase by members of Neissera | Quadferm + (biometrieux Vitek, Hazelwood M0) |
| Quadferm (indicator) | buffered phenol red |
| flat smooth glistening to white colonies, rapid superoxol (+) positive for glucose and oxidase | N. gonorrheae |
| flat smooth glistening colonies from gray to white, weakly (+) for superoxol | N. meningitidis |
| member of Neisseria : oxidase and DNase (+) | M. catarrhalis |
| hard chancre | N. gonorrheae |
| opthalmia neonatorum, urethritis for men and PID for women | N. gonorrheae |
| opthalmia neonatorum treatment | Erythromycin drops |
| Pili, IgA 1 protease with endotoxin LPS | N. gonorrheae |
| meningococcemia or | Waterhouse - Friderichsen syndrome |
| Neisseria that exhibits capsular or Quellang reaction | N. meningitidis |
| Neisseria that is part of normal flora that will cause Otitis media | Moraxella catarrhalis |
Created by:
oddreann
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