click below
click below
Normal Size Small Size show me how
GRAM POS. COCCI
The Lord will bless me! Aja!
Question | Answer |
---|---|
production of bubbles | effervescence |
catalase positive | S. aureus |
catalase negative | Streptococcus |
colonizes skin rarely cause infections | micrococcus |
marine environment | planococcus |
emerging opportunistic infections | stomatococcus |
significant pathogen | staphylococcus |
lysostaphin resistant | micrococcus |
bacitracin resistant | staphylococcus |
aerobic growth | micrococcus |
anaerobic growth | staphylococcus |
modified oxidase positive | micrococcus (oxidizer) |
glucose fermentation | staphylococcus |
agar specialized for S. aureus | MSA (Mannitol Salt Agar) |
concentration of NaCl for MSA | 7.5 % |
MSA: growth with fermentation | yellow zone around colonies (halo) |
MSA: growth without fermentation | red zone around colonies |
MSA: indicator | phenol red |
inhibits gram neg bacteria | PEA blood agar ( phenylethyl alcohol) |
useful to isolate staph and strep blood agar based; inhibits gram neg bacteria | CNA (Colistin Nalidixix Acid) Agar |
CNA: disrupts cell membrane of gram (-) | Colistin |
Colistin or | Polymyxin B |
CNA: blocks DNA replication and membrane integrity in many gram (-) | Nalidixic Acid |
enhances pigmentation of Staph | Loeffler's Serum Slant |
LSS: golden yellow | S. aureus |
LSS: lemon yellow | S. citreus |
LSS: porcelaine white | S. albus |
uses oxacillin + 2 to 4% NaCl to promote growth of resistant strain | MRSA |
indicates oxacillin resistant | MRSA |
for ID of MRSA | chrom agar |
Chrom agar positive color | mauve color |
chrom agar contains | cefoxitin |
coagulase converts soluble fibrinogen to soluble fibrin | coagulase test |
detects cell bound coagulase | slide method |
cell bound coagulase or | clumping factor |
reagent required for coagulase test | Rabbit's plasma |
positive for coag test | clot formation |
detects free coagulase | tube method |
tube method pos reaction | gel like fibrin clot (after 4 hrs) |
utilizes carbohydrate either by respiration or fermentation | OF Glucose Test |
OF: acid in open tube only | oxidizers |
OF: acid in both tubes | Fermenters |
OF: no acid in both tubes | non utilizers |
detects cytochrome oxidase which participates in electron transport | Oxidase Test |
Oxidase test or | Kovac's |
final product of oxidase test | indophenol |
pos color for oxidase test | dark blue |
neg color for oxidase test | no color |
oxidase test by addition of Dimethyl sulfoxide (DMSO) | Modified oxidase - Mibrodase disk |
pos color for modified oxidase | blue color |
MO-MD : VP (+) | S. aureus and S. schleferi |
MO-MD : Pyrase (+) | S. intermedius and S. schleferi |
resistant to 5ug novobiocin | S. saphropyticus |
sensitive to 5ug novobiocin | S. epidermidis |
beta hemolysis with golden yellow pigment | S. aureus |
Beta lactam testing or | Nitrocefin based testing |
(+) for BLT | disk diffusion - zone with clear sharp zone at the edge of disk (CLIFF) |
(-) for BLT | fuzzy zone BEACH edge |
Panton Valintine substances | Leukocidins |
associated with coagglutination in serological tests | Protein A ( S. aureus) |
Scalded Skin syndrome for S. aureus or | Ritter-Lyell Disease |
Toxic shock syndrome nd Gastroenteritis | S. aureus |
Nosocomial infections Prosthetic heart valves Sepsis for IV lines | S. epidermidis |
Highly antibiotic resistant | S. epidermidis |
Second to E. coli to cause UTI | S. saphropyticus |
tampoon or honeycomb cystitis | S. saphropyticus |
lancet shaped | S. pneumoniae |
based on group specific cell wall polysacharride ( C carbohydrate) | Lancefield classification |
Group A beta hemolytic | S. pyogenes |
Group B beta hemolytic | S. agalactiae |
Group D alpha, beta or none | Enterococcus - S. faecalis |
Group D alpha or none | Non- enterococcus - S. bovis |
Alpha hemolytic | S. pneumoniae |
Alpha or Gamma hemolytic | S. viridans |
small zone of hemolysis surrounded by a zone of beta hemolyis | alpha prime hemolysis |
less inhibitory; charac of clear hemolysis produced by beta hemolytic strep | Difibrinated BA |
first choice blood | sheep's blood |
2nd choice blood ( more of haemophilus will show hemolysis) | horse blood |
third choice (production of methemoglobin) | rabbit's blood |
avoided blood | human blood |
test for Group A | PYR hydrolysis test |
PYR hydrolysis test color | red/ pink color |
tests for group B | Hippurate Hydrolysis Test and CAMP test |
Hippurate hydrolysis test pos color | purple complex ( hippuric acid and ninhydrin) |
detects CAMP factor | CAMP TEST |
pos for CAMP test | arrowhead hemolysis |
CAMP means | Christie, Atkins, Munch and Peterson |
test for group D | Bile Esculin Test |
Bile Esculin test ( 40% bile) pos color | Black complex (Esculin to Esculitin binds to ferric chloride) |
test for S. pneumoniae | Bile solubility test |
Bile solubitity test uses | 10% sodium desoxycholate |
detects autolytic amidase that hydrolyzes the peptidoglycan cell wall layer | Bile solubility Test |
pos for PYR hydrolysis Test | Group A and Group D enterococcus |
S. pneumoniae is sensitive to | Vanco, Opto and Baci |
all streptococci are sensitive to | Vancomycin |
scarlet fever | S. pyogenes |
injection of of a skin test dose of erythrogenic toxin | Dick Test |
for confirmation of scarlet fever caused by S. pyogenes | Dick Test |
to detect encapsulated S. pneumoniae | quellang reaction |
are elevated after group A strep infections | ASO titer |
cysteine or | 0.001% pyridoxal (vit B6) |
needs cysteine | nutritionally variant strep |
thiol requiring strep | satelliting strep |
satelliting streps | S. adjacens and S. defectivu |
flesh eating bacteria | S. pyogenes |
for rheumatic fever | Class 1 M protein |
associated with AGN | Class 1 and 2 M protein |
M protein is for | S pyogenes |
mediate the post strep diseases | M protein |
Lipoteichoic acid adherence factor is for | S. pyogenes |
Neonatal infections, Meningitis, pneumonia and Sepsis | S. agalactiae |
Elderly Meningitis, Sepsis or Otitis media | S. pneumoniae |
dental carries (S. mutans) brain or liver abscess ( S. intermedius) | S. viridans |
Extracellular dextran which helps them bind to heart | S viridans |
Acute Bacterial Endocarditis | S. aureus |
Subacute Bacterial Endocarditis | S. viridans and Group D strep |
nucleic acid testing in detection of MRSA | PCR |