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What is the difference between the MIC and the MBC? MIC (minimal inhibitory concentration) = the lowest concentration of an antibiotic that inhibits bacterial growth; MBC (minimal bacteriocidal concentration) = the lowest concentration of an antibiotic with 99.9% killing effect
If a "trailing effect" occurs, how should the MIC be read? What antibiotics is this usually observed with? The lowest concentration which when compared to the growth well shows: (1) approximately 80% reduction of growth, (2) a white button which is less than 2 mm in diameter, (3) a semi-translucent white button - Usually observed w/ sulfonamides (T/S, T, Sx)
What do you do if the GLU well is negative after a 24 hr. incubation? Check if (1) ARG and OF/G or ARG and CET, (2) LYS or (3) ORN are positive. If these are negative, then record MICs on panel first and re-incubate for an additional 24 hrs.
The color for a positive esculin reaction is ______________ and for a positive arginine reaction is _____________. Brown to black; purple
True/False. The control well and the growth well both need to show growth (turbidity) before reading an MIC plate. False. Only the growth well should show growth, the control well should have no growth (CLEAR).
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