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Histology

Staining

TermDefinition
What structures are attracted to a base-like stain? Terminology? Acidic structures. basophilic
What are examples of basophilic structures? Nucleic acids aka dna and rna. Organic Acids. Negatively charged structures.
Basophilic structures stain? blue-purple color
What structures are attracted to a acid-like stain? Terminology? basic structures. Acidophilic
What are examples of acidophilic structures? collagen fibers, cytoplasmic proteins, mitochondria (membrane proteins)
Acidophilic structures stain? pink-red color
The most common basophilic (base like stain) stain? Hematoxylin
The most common acidophilic (acid like stain) stain? Eosin
What specialized stain for chemical properties stain collagen fibers? Color? Trichrome. blue or green .
What specialized stain for chemical properties stain carbohydrate-rich structures? Color? Periodic acid-Schiff (PAS). Magenta
What are examples of carbohydrate-rich structures? glycocalyx, glycosylated proteins.
what stains carbohydrate-rich structures? Periodic acid-schiff - magenta
what stains stain collagen fibers? Color? Trichrome. blue-green.
What is the specialized stain for glycosylated reticular fibers? Color? Silver stain. Black.
What is the specialized stain for elastic fibers/fibroblasts? Color? Orcein. Black or brown.
What makes lipids hard to stain? They are normally lost during sectioning process. (dehydration with alcohol). Lipid rich areas look EMPTY aka fat cells. Two special techniques.
What are two stain names for lipids? Sudan Red (stains red). and Osmic acid (stains black).
What is osmic acid used to stain? Lipids
How are specimens prepared before viewing with microscope? 4 steps. Fixation. Embedding. Thin sectioning. Staining.
Maximum resolving power of a light microscope? Limited by? 0.2 micrometers. (um). Wavelength or energy of light.
Maximum resolving power of an electron microscope? Can improve resolution/max resolution by? 3 nm. Making the wavelength of the electron beam shorter.
Transmission electron microscopy images show what kind of structure? Internal structure.
Scanning electron microscopy images show what kind of structure? External surface of structure.
Created by: Marissafuller
 

 



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