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SCHC Microbiology II
microlicious
| Question | Answer |
|---|---|
| List general characteristics of enterobacteriaceae. | Gram neg rods, catalase pos, oxidase neg, glucose fermenters |
| What are the surface antigens on enterobacteriaceae? What are they associated with? | K--> capsular O-->somatic H-->flagellar |
| Of the Enterics name the true pathogens. | Yersinia, Salmonella, Shigella, and E. coli. |
| When you see a clear red colony with a black center on XLD, what organism is likely? | Salmonella spp. |
| Enlarged lymphnodes can be caused by which enterobacteraceae? What is the name of the enlarged lymph nodes when associated with this organism? | Yersinia pestis, bubo |
| What is HUS? What organism is associated with this condition and the subgroup of the infection. | Hemolytic uremic symdrome; caused by E. coli, EHEC=enterohemorrhagic E. coli. |
| What strain is associated with EHEC? | O157:H7 |
| Name a virulence factor for EHEC | Shiga toxin |
| **add card about sorbitol and o157 | *** |
| How is EHEC transmitted and what is a symptom of the illness. | Undercooked ground beef and raw milk (unpasteurized) |
| What is ETEC? What is it's primary virulence factor? | enterotoxigenic E. coli; contains a enterotoxin similar to V. cholerae. |
| What is the EIEC and what is the main symptom? | Enteroinvasive E. Coli; causes dysentery via direct penetration, invasion, and then destruction of intestinal mucosa. |
| What is the virulence factor of EIEC and what other organism acts similarly? | Direct invasion of the enterocytes; similar action in Shigella |
| What is commonly seen in the stool of people with dysentery? | Mucus and blood |
| ETEC causes what illness? | Traveler's diarrhea or childhood diarrhea. |
| What is EPEC? What is the virulence factor? | enteropathogenic E. coli; organism attaches to the cell surface and causes changes in the cell surface. |
| What is a common symptom in patients with EPEC? | Diarrhea with mucoid stools (blood but not large amounts) |
| WHat is EggEC? What is the virulence factor and the symptoms? | enteraggregative E. coli; organism adheres to mucosal surface; watery diarrhea |
| Which two species of Klebsiella are we concerned with? | K. oxytoca and K. pneumoniae |
| What type of pathogen is K. oxytoca and K. pneumo? What infections can they cause? | Opportunistic; causes bacterial pneumonia in alcoholics, wound infections, UTI, bacteremia |
| What is the colony morphology of klebsiella? | very mucoid tot he point that it can drip onto the lid if inverted. |
| What is the virulence factor for klebsiella? | The polysaccharide capsule. |
| **What drug is klebsiella sp resistant to?** | Ampicillin |
| What kind of infections can enterobacter cause and what type of pathogen is it? | wound infections, UTI, bacteremia, meningitis, respiratory tract infections; oppurtunistic. |
| What drugs are enterobacter species resistant to? | Ampicillin + 1st and 2nd generation cephalosporins |
| Which two species are we concerned with in the clinical lab? | E. aerogenes, E. cloace |
| On the plate which other organism does enterobacter resemble? | Klebsiella |
| What is CIN media selective for? | Yersinia |
| When a disease is transmitted by animals it is called what? | Zoonoses |
| What test can differentiate K. oxytoca and K.pneumo? | Indole+ = K. oxytoca Indole- = K. pneumo |
| What is HE selective for? What does the plate look like when the organism selected for is present? | gram-neg non-fermentors; green |