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Order of Draw
| Blood cultures | Yellow (SPS) | First to minimize chance of microbial contamination | |
| Coagulation tubes | Light blue | Second or third; must be first additive tube because all other additive tubes affect coagulation tests | |
| Plain Non Additive tubes | Red | Prevents contamination by additives in other tubes must be drawn prior to a light blue top as a throw away tube if not ordered | |
| Serum separator gel tubes | Red and grey : gold Plastic | Comes after specimens drawn for coagulation tests because it contains silica particles to activate clotting in the tube | |
| Plasma tubes and plasma separator gel tubes | Green and light green top | Contains heparin, affects coagulation tests and interferes in collection of serum specimens and causes least interference in other tests | |
| EDTA tubes | Lavender, white | Have more carry over (EDTA) problems than any other additive. Elevated sodium and potassium levels. Decreases calcium and iron levels | |
| Oxalate/Fluoride tubes | Gray | Comes after hematology tubes because oxalate damages cell membranes and causes red blood cell morphology | |
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