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MLT Micro
Micro bacteria and the tests for them
| Question | Answer |
|---|---|
| Beta hemolysis is | a large area of hemolysis around a bacterial colony |
| Alpha hemolysis is | a small area of hemolysis around a bacterial colony, usually green in color |
| Gamma hemolysis is | an absence of hemolysis around a bacterial colony |
| A dark pink colony on a MacConkey plate is a | lactose fermenter |
| Staphylococcus aureus is coagulase... | positive |
| Staph epidermisis, Staph saprophyticus, Staph lugdenensis are coagulase... | negative |
| Staphylococcus is Gram ________ _________ | positive cocci |
| Micrococcus is Gram ________ _______ | Positive cocci |
| S.aureus an cause what kind of minor infections | localized skin infections |
| S.aureus is catalase _____ and coagulase____ | positive, positive |
| catalase test reagent | 3% hydrogen peroxide |
| coagulase test reagent | rabbit plasma |
| normal flora on the skin us coagulase | negative |
| coagulase negative staphylococcus is ________ hemolytic | gamma (not) |
| S.sparophyticus is resistant to what antibiotic | novobiocin |
| Micrococcus is catalase _______ and coagulase______ | positive, negative |
| common species of micrococcus are | M. luteus, and M.roseus |
| micrococcus colonies are typically what color | yellow |
| micrococcus are oxidase | positive |
| micrococcus is bacitracin | susseptible |
| Streptococcaceae is Gram __________ ________ | positive cocci |
| Stretococcaceae is catalase | negative |
| S.pyogenes has ____ hemolysis, it is enhanced by ____ conditions | beta, anaerobic |
| S.pyogenes colony appearance on 5% SBA is | small, gray-white |
| S.pyogenes is PYR | positive |
| S.pyogenes is in Lancefield group | Lancefield group A |
| S. agalactiae is in Lancefield group | Lancefield group B |
| S.agalactiae can cause infections in | fetuses and infants |
| In adults S.agalactiae can cause infections | in post partum women |
| S.agalactiae needs to grow in what kind of air | 5% Co2 or ambient air |
| S.agalactiae is catalase | negative |
| S.agalactiae is PYR | negative |
| S,agalactiae is CAMP | positive |
| S,agalactiae hydrolyzes | hippurate |
| S.pneumoniae grows in what kind of air | 5-10% CO2 |
| S.pneumoniae is catalase | negative |
| S.pneumoniae is optochin | susceptible |
| S.pneumoniae is bile | soluble |
| S,pneumoniae is resistant to | penicillin and cephalosporins |
| Viridans streptococci is ________ hemolytic | alpha |
| V.streptococci will grow in what conditions? | 5% CO2 or ambient air |
| V.streptococci is catalase | negative |
| V.streptococci is optochin | resistant |
| V.streptococci is bile | insoluble |
| V.streptococci is bile esculine | negative |
| Enterococcus is ______ hemolytic | alpha or gamma |
| Enterococcus is catalse | negative |
| enterococcus is optochin | resistant |
| enterococcus bile | insoluble |
| enterocuccus is bile esculin | positive |
| enterococci are PYR | positive |
| E.faecium can be resistant to | Vancomycin (VRE) |
| Group D strepococci includes | S.bovis |
| group D streptococci is susceptible to | penicillin |
| The O antigen on enterobacteriaceae is | in the cell wall, and is heat stable |
| the H antigen in enterobacteriaceae is | in the flagella, and is heat labile |
| the K antigen in enterobacteriaceae is | in the polysaccharide capsule, and is heat libile |
| E. coli is usualy _______ hemolytic | beta |
| E.coli on MacConkey is | a lactose fermenter and is dry |
| E.coli is indole | positive |
| E.coli is H2S | negative |
| E.coli is citrate | negative |
| e.coli is susceptible to | most antibiotics |
| Shigella is not a part of | normal flora |
| Shigella is H2S | negative |
| Shigella is able, or unable to move? | non motile |
| shigella is urea | negative |
| shegella on MacConkey | is a non lactose fermenter, but will grow |
| shigella on HE | is a green colony |
| shigela on XLD | is a red colony |
| citrobacter is a part of | normal intestinal flora |
| citrobacter on MacConkey | often a lactose fermenter |
| citrobacter on XLD and HE | forms yellow colonies |
| citrobacter is H2S | variable |
| citrobacter is citrate | positive |
| citrobacter is indole | variable |
| citrobacter is ampicillin | resistant |
| citrobacter is resistant to what kinds of cephalosporins | 1st generation |
| klebsiella/enterobacter group all have what kind of capsules | lare mucoid |
| klebsiella/enterobacter on MacConkey | light pink |
| klebsiella/enterobacter is VP | positive |
| klebsiella/enterobacter is citrate | positive |
| klebsiella/enterobacter is H2S | negative |
| klebsiella/enterobacter is indole | variable |
| klebsiella/enterobacter is urea | variable |
| klebsiella/enterobacter is resistant to | ampicillin |
| klebsiella/enterobacter is resistant to what kinds of cephalosporins except pantoea | 1st generation |
| klebsiella/enterobacter is most susceptible to | trimethoprim/sulfa |
| proteus/providencia on MacConkey | non-lactose fermenter |
| proteus/providencia is PAD | positive |
| proteus/providencia is VP | negative |
| proteus/providencia is H2S and indole | variable |
| proteus/providencia is always resistant to | nitrofuantoin and tetracycline |
| proteus/providencia is or is not motile | very motile |
| Edwardsiella tarda on MacConkey | non lactose fermenter |
| Edwardsiella tarda urea and citrate | negative |
| Edwardsiella tarda H2S and indole | positive |
| Edwardsiella tarda probable cause of | diarrhea |
| Ewingella americana on MacConkey | lactose fermenter |
| Ewingella americana is typically what kind of pathogen | plant |
| Hafnia alvei on MacConkey | non-lactose fermenter |
| Hafnia alvei is VP and citrate | positive |
| salmonella on MacConkey, HE, and XLD | nonfermenter |
| salmonella is indole, VP, and urea | negative |
| salmonella is H2S | positive |
| Plesiomonas is a member of | enterobacteriacae |
| Plesiomonas is oxidase | positive |
| Plesiomonas is a glucose and lactose | fermenter |