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Unit 4 Chapter 20

Vibrio , Aeromonas , Plesiomonas and Campylobacter Species

Vibrio *colony morphology* polar, sheathed flagella, curved, highly polymorphic
Vibrio *gram stain results* gram- negative rods
Vibrio *testing results* oxidase-positive, catalase-positive, reduces nitrate to nitrites, positive mucoid stringing
Vibrio *virulence factors* common flagellar, H antigens, somatic o antigens
Vibrio *epidemiology* aquatic environments including fresh water, brackish or esturine water, and marine or salt water.
Vibrio *infections* gastroenteritis, cholera, fatal septicemia, necrotizing fasciitis
V cholera *colony morphology* medium to large smooth opaque, iridescent with greenish hue, α or β- hemolytic
V cholera *gram stain results* gram-negative curved rods
V cholera *testing results* VP positive, Hemolyzes, erythrocytes, inhibited by polymyxin B
V cholera *virulence factors* resistance to tetracycline, doxycycline
V cholera *epidemiology* developing countries, Bengal, Bangladesh, asia and Africa, central America
V cholera *infections* cholera, gastroenteritis, motile, single polar flagellum
Aeromonas *colony morphology* mesophilic or psychrophilic, large round raised opaque, smooth and often mucoid
Aeromonas *gram stain results* Gram negative rods
Aeromonas *testing results* Oxidase positive, Glucose Fermenting
Aeromonas *virulence factors* multifactorial patterns associated with enteric disease
Aeromonas *epidemiology* fresh water, estuarine. Marine environments
Aeromonas *infections* pediatric diarrhea, gastrointestinal infection, inflammatory bowel disease, extra intestinal infections
Plesiomonas *colony morphology* Motile by two to five lophotrichous polar flagella; swarming does not occur; Colonies appear grayish, shiny, opaque with a smooth surface.
Plesiomonas *gram stain results* Gram-negative. Round ended, straight rods
Plesiomonas *testing results* Oxidase-positive. Catalase-positive. Lysine-, Ornithine-, and Arginine-Decarboxylase-positive. Indole-positive. Lipase-negative. Most strains are sensitive to vibriostatic agent 0/129. DNase-negative. Voges-Proskauer-negative.
Plesiomonas *virulence factors* resistance to penicillin class of antibiotics and more than one amnioglycoside.
Plesiomonas *epidemiology* soil and aquatic environments; widely distributed among warm- and cold-blooded animals
Plesiomonas *infections* gastroenteritis, extra intestinal infection
Campylobacter *colony morphology* curved, non-spore-forming, moist runny looking and spreading, usually nonhemolytic, round raised or flat; can produce smooth convex translucent colonies.
Campylobacter *gram stain results* gram-negative rods
Campylobacter *testing results* jejuni: catalsae-positive, reduces nitrate to nitrite, urease- negative, H2S - negative, hippurate hydrolysis -positive, Indoxyl acetate hydrolysis - positive
Campylobacter *virulence factors* multiple flagella
Campylobacter *epidemiology* direct contact with infected pets, indirectly by consumption of contaminated water, dairy products, and improperly cooked poultry, some spp. are sexually transmitted
Campylobacter *infections* diarrheal disease, nausea, vomitting
Helicobacter pylori *colony morphology* convex and translucent in appearance, slight hemolysis around colonies, which are grey in color.
Helicobacter pylori *gram stain results* Gram-negative, microaerophilic
Helicobacter pylori *testing results* catalase - positive, variable nitrate reduction, urease - negative, TSI - negative, Hippurate Hydrolysis - negative, Indoxyl Acetate Hydrolysis - negative
Helicobacter pylori *virulence factors* strong ureolytic ability, sheathed flagella
Helicobacter pylori *epidemiology* gastric or intestinal sites in mammalian host animals.
Helicobacter pylori *infections* gastric, peptic. and duodenal ulcers as well as gastrointestinal carcinoma
Created by: luceroapril