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Unit 3 Chapter 17

Neisseria Species and Moraxella catarrhalis

QuestionAnswer
Neisseria *colony morphology* aerobic, nonmotile, non-sporeforming
Neisseria *gram stain results* gram-negative diplococci
Neisseria *virulence factors* receptors for human transferrin, capsule, pili, cell membrane protein, lipooligosaccharide or endotoxi, IgA
Neisseria *epidemiology* mucus membranes of the respiratory and urogenital tract
Neisseria gonorrhoeae *colony morphology* aerobic, can demonstrate motility; chocolate agar: small, gray or tan, translucent, and raised after 24 to 48 hrs of incubation
Neisseria gonorrhoeae *gram stain results* gram-negative diplococcus
Neisseria gonorrhoeae *testing results* produces acid only from glucose in the carbohydrate acidification test
Neisseria gonorrhoeae *virulence factors* surface antigens
Neisseria gonorrhoeae *epidemiology* most commonly transmitted by sexual contact
Neisseria gonorrhoeae *infections* pelvic inflammatory disease, ophthalmia neonatrum, proctitis, pharyngitis, conjunctivitis, peritontis, and perihepatitis
Oxidase test *principle* used to identify bacteria containing the respiratory enzyme cytochrome c oxidase.
Oxidase test *results reporting* positive-purple blue color; negative-no color
Modified Thayer Martin agar *principle* utilized for primary isolation of Neisseria meningitidis from mixed specimens. The agar is classified as a selective enrichment agar.
Modified Thayer Martin agar *reagents* Enrichments added to this medium include both X and V factors; includes more agar to help prevent swarming Proteus. The agar contains antibiotics to inhibit the growth of normal flora, non-pathogenic Neisseria species and most other organism
CTA testing with lactose, sucrose, maltose, xylose, ect *principle* CTA Media are recommended for the determination of carbohydrate fermentation by fastidious organisms such as Neisseria spp. They are also used for the detection of bacterial motility and serve as a culture media for microorganism maintenance
CTA testing with lactose, sucrose, maltose, xylose, ect *reagents* 1% concentration of a specific carbohydrate used to detect fermentation reactions; decreasing possibility of reversal reactions from carbohydrate depletion, masking the acid by-products w/alkaline by-products with peptone degradation this causes a decre
CTA testing with lactose, sucrose, maltose, xylose, ect *results reporting* A positive carbohydrate fermentation reaction is the development of a yellow color change. A negative carbohydrate fermentation reaction is demonstrated by a red-pink or deep red color in the medium.
Neisseria meningitis *colony morphology* aerobic, some motility
Neisseria meningitis *gram stain results* gram-negative diplococcus
Neisseria meningitis *testing results* produces acid from glucose and maltose in the carbohydrate acidification test
Neisseria meningitis *virulence factors* surface pili, heavy capsule, hemolysin, surface components
Neisseria meningitis *epidemiology* mucosal surfaces of the nasopharynx, oropharynx, and anogenital region
Neisseria meningitis *infections* fulminant sepsis and meningitis
Moraxella catarrhalis *colony morphology* smooth, opaque, gray to white colonies
Moraxella catarrhalis *gram stain results* gram-negative diplococci
Moraxella catarrhalis *testing results* asaccharolytic differentiated by a positive DNase and butyrate esterase reactions
Moraxella catarrhalis *virulence factors* β-lactamase
Moraxella catarrhalis *epidemiology* opportunistic pathogen
Moraxella catarrhalis *infections* upper respiratory tract infection, lower repiratory infections, acute otitis media and sinusitis in children
other Neisseria species and their identifiers traditional pathogens, commensal that can grow on selective media, commensal that do not usually grow on selective media. N. cinerea, N. flavescens, N. lactamica, N. mucosa, N. polysaccharea, N. sicca, N. subflava, N. elongata, and N. weaveri
Created by: luceroapril