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Semen Analysis
MEDT 208 Urinalysis Semen Analysis
An increased amount of prostatic fluid in a semen specimen will: | lower the pH |
Evaluation of sperm motility includes: | Examination in 1 hour, and estimation of progressive motion in 20 fields or 100 sperm |
In addition to the sperm count in a fertility study, analysis of seminal fluid should also include: | time of liquefaction, estimation of motility, morphology |
Sperm can be immobilized before performing a sperm concentration using all of the following except: | Heating to 37º C |
When should semen specimens should be analyzed for viscosity? | After liquifaction. |
The sugar of interest in cases of infertility is: | Fructose |
To determine if a questionable specimen is semen, the specimen should be tested for: | Acid Phosphatase |
Using a 1:20 dilution of semen, a student counts 70 sperm in the five RBC squares on one side of the Neubauer hemocytometer and 82 sperm on the other side. The student should: | Repeat dilutions AND Repeat the count |
Which parameter of the semen analysis is most critically affected if the first portion of the ejaculation is not collected? | Sperm concentration |
Yellow color in a semen specimen may be caused by all of the following except: | Preservation at room temperature. |
Yellow color in a specimen CAN be caused by: | Prolonged abstinence, Medications, Urine |
The normal pH for semen specimens is? | 7.2-8.0 |
What is the standard for normal sperm morphology? | > 30% normal is ok |
Which squares in the Neubauer chamber are counted for semen analysis? | 5 center squares |
What is the normal concentration for sperm? | 20-160 million/mL |
What is the standard dilution for sperm analysis? | 1:20 |
What is normal agglutination for semen analysis? | no agglutination |
What is the principle of the Mixed agglutination reaction (MAR) test? | Incubate sperm w/antihuman globulin (AHG) and IgG-coated latex particles AHG combines w/particles & antibody-coated sperm forming clumps. |
What is the principle of the Immunobead Test? | Demonstrates antibodies to head, neck, midpiece, and tail. Beads are coated with antihuman globulin |
The Eosin-nigrosin stain and Seminal fructose tests are tests for? | Viability |
What color are normal cells in the Eosin-nigrosin stain? | blue-white |
What are the three sections of a sperm? | Head, neckpiece, tail |